手性分析;中药与天然药物质量控制;有机小分子及体内药物分析;多肽及反义核酸药物研究与评价
商品化的手性色谱柱介绍(二)
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下一篇 2008-01-25 20:43:03
Chiral Analytical HPLC Columns
Eka Chemicals offer two chiral phases: Kromasil CHI-DMB & CHI-TBB, in which acylated N,N'-diallyl-l-tartardiamide network polymers have been covalently bound to Kromasil.
Kromasil CHI-DMB |
|
|
0,0'-bis (3,5-dimethylbenzoyl)-N,N'-diallyl-L-tartardiamide |
|
5µm & 10µm |
|
340m2/g |
|
100Å |
|
14.5% |
|
0.6% |
Kromasil CHI-TBB |
|
|
0,0'-bis (4-tert-butylbenzoyl)-N,N'-diallyl-L-tartardiamide |
|
5µm & 10µm |
|
340m2/g |
|
100Å |
|
15.0% |
|
0.6% |
Two key steps are involved in their synthesis. The chiral monomers are reacted with a multifunctional hydrosilane yielding a network polymer incorporating a bifunctional C2-symmetric chiral selector. The chiral polymer is covalently bonded to functionalised Kromasil silica.
Description |
Quote Part No. |
Kromasil 5µm CHI-DMB 250 x 4.6mm |
KR100-5CHI-DMB-250A |
Kromasil 5µm CHI-TBB 250 x 4.6mm |
KR100-5CHI-TBB-250A |
Kromasil 10µm CHI-DMB 250 x 4.6mm |
KR100-10CHI-DMB-250A |
Kromasil 10µm CHI-TBB 250 x 4.6mm |
KR100-10CHI-TBB-250A |
ChromTech formally of Sweden, now based in the UK, manufacture 3 specific phases for the HPLC separation of chiral compounds.
- spherical porous silica
- reversed-phase applications
- unique separation characteristics
- 150+ cited references
- over 200 chiral molecules resolved
Phase |
Chiral selector |
Particle size (µm) |
Application |
CHIRAL-AGP |
α1-acid glycoprotein |
5 |
broad: amines; acids; alcohols; esters; sulphoxides |
CHIRAL-CBH |
cellobiohydrolase |
5 |
alcohols; phenols; carbonyls; amides; ethers; esters |
CHIRAL-HSA |
human serum albumin |
5 |
weak acids; strong acids; zwitterionic compounds |
Solution pH, organic solvent modifier & aqueous buffer affect the performance of the columns.
Parameter |
Typical conditions |
Effect of varying parameter |
pH |
4 - 7 |
variable |
buffer |
phosphate 0.01 - 0.1M |
increase in buffer concentration can increase retension & enantioselectivity |
organic solvent |
propan-2-ol, acetonitrile,methanol 5-15% |
Selection of solvent strongly affects enantioselectivity. Higher organic solvent ratios reduce retension time for all phases. For both AGP & HSA columns, enantioselectivity is simultaneously reduced, whilst for CBH columns it is often increased. |
Semi prep columns available without discount
Phase |
Column dimensions |
100x2 |
150x2 |
100x3 |
150x3 |
50x4 |
100x4 |
150x4 |
CHIRAL-AGP |
AGP100.2 |
AGP150.2 |
AGP100.3 |
AGP150.3 |
AGP50.4 |
AGP100.4 |
AGP150.4 |
CHIRAL-CBH |
CBH100.2 |
CBH150.2 |
CBH100.3 |
CBH150.3 |
CBH50.4 |
CBH100.4 |
CBH150.4 |
CHIRAL-HSA |
HSA100.2 |
HSA150.2 |
HSA100.3 |
HSA150.3 |
HSA50.4 |
HSA100.4 |
HSA150.4 |
DACH-DNB chiral stationary phase
The DACH-DNB CSP has the following advantages:
- column durability due to covalent bonding
- ability to invert elution order
- excellent chromatographic efficiency
- universal solvent compatability
Cat # |
Description |
788101 |
(R,R)-DACH-DNB 25cm x 4.6mm 5µm 100Å |
788102 |
(R,R)-DACH-DNB 25cm x 10.0mm 5µm 100Å |
788201 |
(S,S)-DACH-DNB 25cm x 4.6mm 5µm 100Å |
788202 |
(S,S)-DACH-DNB 25cm x 10.0mm 5µm 100Å |
The DACH-DNB material is also available for large-scale preparative separation
Cat # |
Description |
786201 |
(R,R)-Whelk-O 1 25cm x 4.6mm 5µm 100Å |
786101 |
(S,S)-Whelk-O 1 25cm x 4.6mm 5µm 100Å |
786515 |
(R,R)-Whelk-O 1 25cm x 4.6mm 10µm 100Å |
786615 |
(S,S)-Whelk-O 1 25cm x 4.6mm 10µm 100Å |
786315 |
(R,R)-Whelk-O 2 25cm x 4.6mm 10µm 100Å |
786415 |
(S,S)-Whelk-O 2 25cm x 4.6mm 10µm 100Å |
787200 |
(R,R)-ULMO 25cm x 4.6mm 5µm 100Å |
787100 |
(S,S)-ULMO 25cm x 4.6mm 5µm 100Å |
731044 |
(3R,4S)-Pirkle 1-J 25cm x 4.6mm 5µm 100Å |
731045 |
(3S,4R)-Pirkle 1-J 25cm x 4.6mm 5µm 100Å |
731043 |
(R,R)-ß-Gem 1 25cm x 4.6mm 5µm 100Å |
781029 |
(S,S)-ß-Gem 1 25cm x 4.6mm 5µm 100Å |
785035 |
(R,R)-α-Burke 2 25cm x 4.6mm 5µm 100Å |
735037 |
(S,S)-α-Burke 2 25cm x 4.6mm 5µm 100Å |
731054 |
D-Leucine 25cm x 4.6mm 5µm 100Å |
731041 |
L-Leucine 25cm x 4.6mm 5µm 100Å |
731021 |
D-Phenylglycine 25cm x 4.6mm 5µm 100Å |
731024 |
L-Phenylglycine 25cm x 4.6mm 5µm 100Å |
731034 |
L-Naphthylleucine 25cm x 4.6mm 5µm 100Å |
来源: http://www.hichrom.co.uk/technical/chiral.htm
The Regis Pirkle-Concept Chiral HPLC Column Its Nature, Care, and Use
http://www.registech.com/chiral/care_and_use.html
http://www.registech.com/chiral/
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