Hydrogen-rich saline reduces delayed neurologic sequelae in
experimental carbon monoxide toxicity
Qiang Sun, Jianmei Cai, Jiangrui Zhou, Hengyi Tao , John H Zhang, Wei
Zhang*, Xuejun Sun*
Objective: We investigated the feasibility and efficacy of hydrogen-rich
saline therapy on delayed neurologic sequelae in rats after severe acute carbon
monoxide (CO) poisoning.
Design: Controlled animal study.
Setting: Research laboratory of a university hospital.
Subjects: Sprague-Dawley rats weighing 250 ± 20 g.
Interventions: The rats were exposed to 1000 ppm CO in air for 40 minutes
and then to 3000 ppm for another 20 minutes until they lost consciousness. Rats
were intraperitoneal injected with hydrogen-rich saline or normal saline
(10ml/Kg) for six times after resuscitation at 0, 12, 24, 36, 48, and 60 hr,
respectively. The rats without CO poisoning were used as normal controls.
Measurements and Main Results: CO poisoning induced brain tissue
inflammation, cell death, and cognitive dysfunction (Morris Water Maze) at one
week after the CO insult. Hydrogen-rich saline treatment significantly
attenuated degraded myelin basic protein (MBP), decreased the expression of
Iba1, a microglial marker, reduced DNA oxidation, and suppressed
pro-inflammatory cytokine interleukin Interleukin-1β (IL-1β)，Interleukin-6
(IL-6) and Tumor necrosis factor-alpha (TNF-a) in the cortex and hippocampal
tissues when compared with those in normal saline-treated rats. These
histological and biological improvements were accompanied with an improvement in
the Morris water maze test.
Conclusions: This observation demonstrated that hydrogen-rich saline
peritoneal injection improves histological and functional assessment of CO
encephalopathy in a rat model. Hydrogen saline has potentials as a novel and
alternative therapy for severely CO-poisoned patients with delayed neurologic
sequelae. The therapeutic effects of hydrogen-rich saline may be related to
antioxidant and anti-inflammatory actions.