这本书是搞光学成像的必不得少的一本教科书,非常经典!!!!!
Fundamentals of Light Microscopy and Electronic Imaging
Douglas B. Murphy
ISBN: 978-0-471-25391-4
Hardcover
384 pages
October 2001
description:
Over the last decade, advances in science and technology have profoundly
changed the face of light microscopy. Research scientists need to learn new
skills in order to use a modern research microscope–skills such as how to align
microscope optics and perform. image processing. Fundamentals of Light Microscopy
and Electronic Imaging explores the basics of microscope design and use. The
comprehensive material discusses the optical principles involved in diffraction
and image formation in the light microscope, the basic modes of light
microscopy, the components of modern electronic imaging systems, and the image
processing operations necessary to acquire and prepare an image.
Written in a practical, accessible style, Fundamentals of Light Microscopy
and Electronic Imaging reviews such topics as:
Illuminators, filters, and isolation of specific wavelengths
Phase contrast and differential interference contrast
Properties of polarized light and polarization microscopy
Fluorescence and confocal laser scanning microscopy
Digital CCD microscopy and image processing
Each chapter includes practical demonstrations and exercises along with a
discussion of the relevant material. In addition, a thorough glossary assists
with complex terminology and an appendix contains lists of materials, procedures
for specimen preparation, and answers to questions.
An essential resource for both, experienced and novice microscopists.
table of content:
Preface.
Fundamentals of Light Microscopy.
Light and Color.
Illuminators, Filters, and Isolation of Specific Wavelengths.
Lenses and Geometrical Optics.
Diffraction and Interference in Image Formation.
Diffraction and Spatial Resolution.
Phase Contrast Microscopy and Dark-Field Microscopy.
Properties of Polarized Light.
Polarization Microscopy.
Differential Interference Contrast (DIC) Microscopy and Modulation Contrast
Microscopy.
Fluorescence Microscopy.
Confocal Laser Scanning Microscopy.
Video Microscopy.
Digital CCD Microscopy.
Digital Image Processing.
Image Processing for Scientific Publication.
Appendix I.
Appendix II.
Appendix III.
Glossary.
References.
Index.