supersensitive BPA ELISA Kit过敏双酚A试剂盒

上一篇 / 下一篇  2010-09-03 11:27:25 / 个人分类:供求

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supersensitive BPA ELISA Kit过敏双酚A试剂盒

 

Bisphenol A (BPA), principal raw material for polycarbonate and epoxy resins, is suspected as one of the endocrine disrupting chemicals.
GC/MS, generally employed methods for quantitative BPA analysis, requires expensive instrumentation as well as complex and time-consuming extraction process with hazardous organic solvent.


This test kit detects BPA in environment or in vitro with high sensitivity with the aid of solid phase extraction.

Features

  • The quantitative analysis ranges from 0.05-10μg/L(ppb), sensitive enough to detect BPA in a specimen from river water, etc..
  • A simple solid phase extraction is available to measure samples with lower concentration.
  • The total time for measurement is only 2.5 hours.
  • BPA monoclonal antibody binds exclusively with BPA and does not show cross-reaction with other chemicals with similar structures.
  • The amount of harmful solvent can be minimized.

Standard Curve of BPA

Cross-Reactivity Pattern

Compounds

Cross Reactivity(%)

Bisphenol A (BPA)

100

 Diethylhexylphthalate (DEHP)

<0.05

 Nonylphenol (NP)

0.19

 17β-Estradiol (E2)

<0.05

 Estrone (E1)

<0.05

 Linear alkylbenzene sulfonate (LAS)

<0.05

 Alkylphenol Ethoxylate (APE)

<0.05

 Alkyl Ethoxylate (AE)

<0.05

 Humic acid Na

<0.05

.

 

Kit Format

Kit Format

Comment

Microplate

1 plate (96 wells)& reagents

Needs a microplate reader (450nm)
For multiple and simultaneous measurement

Simple Solid Phase Extraction (example)

  • Adjust the pH of the sample to 3.5 with 1M HCl.
  • Wash out C18 solid phase cartridge with MeOH and distilled water; then pour the sample through the cartridge.
  • Rinse the cartridge with water, then elute the mixture of aceton and MeOH at the ratio of 1:1.
  • Dry the sample with nitrogen gas, then adjust MeOH concentration at 10% for measurement.

 

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