supersensitive BPA ELISA Kit过敏双酚A试剂盒
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下一篇 2010-09-03 11:27:25/ 个人分类:供求
supersensitive BPA ELISA Kit过敏双酚A试剂盒
Bisphenol A (BPA), principal raw material for polycarbonate and epoxy resins, is suspected as one of the endocrine disrupting chemicals.
GC/MS, generally employed methods for quantitative BPA analysis, requires expensive instrumentation as well as complex and time-consuming extraction process with hazardous organic solvent.
This test kit detects BPA in environment or in vitro with high sensitivity with the aid of solid phase extraction.
Features
- The quantitative analysis ranges from 0.05-10μg/L(ppb), sensitive enough to detect BPA in a specimen from river water, etc..
- A simple solid phase extraction is available to measure samples with lower concentration.
- The total time for measurement is only 2.5 hours.
- BPA monoclonal antibody binds exclusively with BPA and does not show cross-reaction with other chemicals with similar structures.
- The amount of harmful solvent can be minimized.
Standard Curve of BPA |
|
Cross-Reactivity Pattern |
Compounds |
Cross Reactivity(%) |
Bisphenol A (BPA) |
100 |
Diethylhexylphthalate (DEHP) |
<0.05 |
Nonylphenol (NP) |
0.19 |
17β-Estradiol (E2) |
<0.05 |
Estrone (E1) |
<0.05 |
Linear alkylbenzene sulfonate (LAS) |
<0.05 |
Alkylphenol Ethoxylate (APE) |
<0.05 |
Alkyl Ethoxylate (AE) |
<0.05 |
Humic acid Na |
<0.05 |
|
. |
Kit Format
Kit Format |
Comment |
Microplate |
1 plate (96 wells)& reagents |
Needs a microplate reader (450nm) For multiple and simultaneous measurement |
|
|
|
Simple Solid Phase Extraction (example)
- Adjust the pH of the sample to 3.5 with 1M HCl.
- Wash out C18 solid phase cartridge with MeOH and distilled water; then pour the sample through the cartridge.
- Rinse the cartridge with water, then elute the mixture of aceton and MeOH at the ratio of 1:1.
- Dry the sample with nitrogen gas, then adjust MeOH concentration at 10% for measurement.
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