Total Estrogen (E1/E2/E3) ELISA Kit 雌激素试剂盒
上一篇 /
下一篇 2010-09-03 11:27:56/ 个人分类：供求
Total Estrogen (E1/E2/E3) ELISA Kit 雌激素试剂盒
Total Estrogen (E1/E2/E3) ELISA Kit
- ES monoclonal antibody binds exclusively with E1, E2 and E3, and does not show cross-reaction with other chemicals of similar structures. A monoclonal antibody is uniform. in quality, generating very little lot-to-lot variation.
- The quantitative analysis ranges from 0.1μg/L to 3μg/L (ppb).
- The ELISA measurement is highly reproducible; the coefficient of variation (CV) is mostly under 10%.
- The assay requires less amount of harmful solvent than instrument analyses.
- With ease of handling, the total time for measurement is only 2.5 hours.
- The kit, a 96-well microplate format, enables simultaneous measurement of multiple samples at more reasonable cost.
Chemical Structures of Targets
Standard Curves
Pretreatment Methods
See here.
Test Data
|
Recovery Test using River Water (n=4) |
|
|
|
|
ES ELISA Kit |
|
Unspiked |
|
Determination (ng/L) |
12.4 |
|
SD (%) |
6.5 |
|
Spiked with 2ng/L E2 |
|
Determination (ng/L) |
14.4 |
|
SD (%) |
9.2 |
|
Recovery (%) (n=4) |
100.0 |
|
Comparison with LC/MS/MS
|
Comparison with LC/MS/MS |
|
|
|
|
E1+E2 (ng/L) |
|
Sample |
LC/MS/MS |
ES ELISA Kit |
|
A |
2.08 |
3.75(1.8) |
|
B |
4.25 |
5.36(1.3) |
|
|
|
|
Figures in parentheses indicate relative value to LC/MS/MS data. |
Cross-reactivity Pattern
|
Estrogens |
|
|
|
Compound |
% Reactivity |
|
ES ELISA Kit |
|
Estron (E1) |
87.0 |
|
2-methoxy E1 |
<0.03 |
|
E1-3-sulfate |
<0.03 |
|
17β-Estradiol (E2) |
100.0 |
|
16-keto E2 |
118.0 |
|
2-methoxy E2 |
0.2 |
|
E2-17-glucronide |
5.0 |
|
E2-3-glucronide |
<0.03 |
|
E2-3-sulfate-17-glucronide |
0.5 |
|
Ethynylestradiol |
0.7 |
|
Estriol (E3) |
55.0 |
|
16-epi-E3 |
129.0 |
|
E3-16-glucronide |
48.0 |
|
|
Other hormones |
|
|
|
Compound |
% Reactivity |
|
ES ELISA Kit |
|
cis -Androsterone |
<0.03 |
|
trans -Androsterone |
<0.03 |
|
Cholesterol |
<0.03 |
|
Dehydroisoandrosterone |
<0.03 |
|
5α-Dihydroteststerone |
<0.03 |
|
Hydrocortisone |
<0.03 |
|
Pregnenolone |
<0.03 |
|
Teststerone |
<0.03 |
|
Kit Format
|
Kit Format |
Comment |
|
Microplate |
1 plate (96 wells)& reagents |
Needs a microplate reader (450nm)
For multiple and simultaneous measurement |
|
|
|
如有更多其它试剂产品需求与查询,请及时接洽：info@express-cn.com 或在我公司查询主页客户留言处（http://www.express-cn.com/expressgb/lyb.htm ）留下您的查询内容，我们即会在当天为您作出回复。
伊普瑞斯科技有限公司
info@express-cn.com
在线客服：QQ：1069267096
MSN：liyuqiu1983@hotmail.com
cindylee-1981@hotmail.com
联系人：杨先生
总机：010-60206266
传真：010-60206773
www.express-cn.com
导入论坛收藏
分享给好友
推荐到圈子
管理
举报
TAG:
