Total Estrogen (E1/E2/E3) ELISA Kit 雌激素试剂盒

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Total Estrogen (E1/E2/E3) ELISA Kit  雌激素试剂盒

 

Total Estrogen (E1/E2/E3) ELISA Kit

  • ES monoclonal antibody binds exclusively with E1, E2 and E3, and does not show cross-reaction with other chemicals of similar structures. A monoclonal antibody is uniform. in quality, generating very little lot-to-lot variation.
  • The quantitative analysis ranges from 0.1μg/L to 3μg/L (ppb).
  • The ELISA measurement is highly reproducible; the coefficient of variation (CV) is mostly under 10%.
  • The assay requires less amount of harmful solvent than instrument analyses.
  • With ease of handling, the total time for measurement is only 2.5 hours.
  • The kit, a 96-well microplate format, enables simultaneous measurement of multiple samples at more reasonable cost.

 

Chemical Structures of Targets

Standard Curves

 

Pretreatment Methods
See here.

Test Data

Recovery Test using River Water (n=4)

 

 

ES ELISA Kit

Unspiked

Determination (ng/L)

12.4

SD (%)

6.5

Spiked with 2ng/L E2

Determination (ng/L)

14.4

SD (%)

9.2

Recovery (%) (n=4)

100.0

Comparison with LC/MS/MS

Comparison with LC/MS/MS

 

 

E1+E2 (ng/L)

Sample

LC/MS/MS

ES ELISA Kit

A

2.08

3.75(1.8)

B

4.25

5.36(1.3)

 

Figures in parentheses indicate relative value to LC/MS/MS data.

Cross-reactivity Pattern

Estrogens

 

Compound

% Reactivity

ES ELISA Kit

Estron (E1)

87.0

2-methoxy E1

<0.03

E1-3-sulfate

<0.03

17β-Estradiol (E2)

100.0

16-keto E2

118.0

2-methoxy E2

0.2

E2-17-glucronide

5.0

E2-3-glucronide

<0.03

E2-3-sulfate-17-glucronide

0.5

Ethynylestradiol

0.7

Estriol (E3)

55.0

16-epi-E3

129.0

E3-16-glucronide

48.0

 

Other hormones

 

Compound

% Reactivity

ES ELISA Kit

cis -Androsterone

<0.03

trans -Androsterone

<0.03

Cholesterol

<0.03

Dehydroisoandrosterone

<0.03

5α-Dihydroteststerone

<0.03

Hydrocortisone

<0.03

Pregnenolone

<0.03

Teststerone

<0.03

Kit Format

Kit Format

Comment

Microplate

1 plate (96 wells)& reagents

Needs a microplate reader (450nm)
For multiple and simultaneous measurement

 

 

 

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