17β-Estradiol (E2) ELISA Kit 17β雌二醇E2试剂盒
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下一篇 2010-09-03 11:28:23/ 个人分类：供求
17β-Estradiol (E2) ELISA Kit 17β雌二醇E2试剂盒
- E2 monoclonal antibody binds exclusively with E2 and does not show cross-reaction with other chemicals of similar structures. A monoclonal antibody is uniform. in quality, generating very little lot-to-lot variation.
- The quantitative analysis ranges from 0.05μg/L to 1μg/L (ppb).
- The ELISA measurement is highly reproducible; the coefficient of variation (CV) is mostly under 10%.
- The assay requires less amount of harmful solvent than instrument analyses.
- With ease of handling, the total time for measurement is only 2.5 hours.
- The kit, a 96-well microplate format, enables simultaneous measurement of multiple samples at more reasonable cost.
|
Chemical Structure |
Standard Curves |
|
|
|
Pretreatment Methods
See here.
Comparison with LC/MS/MS
|
Averaged value of E2 (ng/L) |
|
Sample |
Na) |
LC-MS/MS |
E2-Ecologienab) |
E2-ADc) |
E2-CCc) |
E2-NGc) |
E2-RBc) |
|
Primary effluent |
10 |
8.4 |
12.5 |
21.1 |
81.4 |
97.6 |
202.2 |
|
Aeration tank |
13 |
1.9 |
2.1 |
4.4 |
9.4 |
7.8 |
31.6 |
|
Secondary effluent |
5 |
1.2 |
2.6 |
5.3 |
15.1 |
18.8 |
75.9 |
|
|
|
a) |
N=number of samples |
|
b) |
Ecologiena E2 kit |
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c) |
Other kits on the market (for biological samples) |
Cross-reactivity Pattern
|
Estrogens |
|
|
|
Compound |
% Reactivity |
|
E2 ELISA Kit |
|
Estron (E1) |
1.3 |
|
2-methoxy E1 |
<0.4 |
|
E1-3-sulfate |
1.0 |
|
17β-Estradiol (E2) |
100.0 |
|
16-keto E2 |
16.0 |
|
2-methoxy E2 |
2.0 |
|
E2-17-glucronide |
<0.4 |
|
E2-3-glucronide |
16.0 |
|
E2-3-sulfate-17-glucronide |
<0.4 |
|
Estriol (E3) |
0.6 |
|
16-epi-E3 |
0.5 |
|
E3-16-glucronide |
<0.4 |
|
|
|
|
Other hormones |
|
|
|
Compound |
% Reactivity |
|
E2 ELISA Kit |
|
cis -Androsterone |
<0.03 |
|
trans -Androsterone |
<0.03 |
|
Cholesterol |
0.46 |
|
Dehydroisoandrosterone |
<0.03 |
|
5α-Dihydroteststerone |
0.38 |
|
Hydrocortisone |
0.38 |
|
Pregnenolone |
0.35 |
|
Teststerone |
<0.03 |
|
Kit Format
|
Kit Format |
Comment |
|
Microplate |
1 plate (96 wells)& reagents |
Needs a microplate reader (450nm)
For multiple and simultaneous measurement |
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|
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