17β-Estradiol (E2) ELISA Kit 17β雌二醇E2试剂盒

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17β-Estradiol (E2) ELISA Kit  17β雌二醇E2试剂盒

  • E2 monoclonal antibody binds exclusively with E2 and does not show cross-reaction with other chemicals of similar structures. A monoclonal antibody is uniform. in quality, generating very little lot-to-lot variation.
  • The quantitative analysis ranges from 0.05μg/L to 1μg/L (ppb).
  • The ELISA measurement is highly reproducible; the coefficient of variation (CV) is mostly under 10%.
  • The assay requires less amount of harmful solvent than instrument analyses.
  • With ease of handling, the total time for measurement is only 2.5 hours.
  • The kit, a 96-well microplate format, enables simultaneous measurement of multiple samples at more reasonable cost.

 

Chemical Structure

Standard Curves

Pretreatment Methods
See here.

Comparison with LC/MS/MS

Averaged value of E2 (ng/L)

Sample

Na)

LC-MS/MS

E2-Ecologienab)

E2-ADc)

E2-CCc)

E2-NGc)

E2-RBc)

Primary effluent

10

8.4

12.5

21.1

81.4

97.6

202.2

Aeration tank

13

1.9

2.1

4.4

9.4

7.8

31.6

Secondary effluent

5

1.2

2.6

5.3

15.1

18.8

75.9

 

 

a)

N=number of samples

b)

Ecologiena E2 kit

c)

Other kits on the market (for biological samples)

Cross-reactivity Pattern

Estrogens

 

Compound

% Reactivity

E2 ELISA Kit

Estron (E1)

1.3

2-methoxy E1

<0.4

E1-3-sulfate

1.0

17β-Estradiol (E2)

100.0

16-keto E2

16.0

2-methoxy E2

2.0

E2-17-glucronide

<0.4

E2-3-glucronide

16.0

E2-3-sulfate-17-glucronide

<0.4

Estriol (E3)

0.6

16-epi-E3

0.5

E3-16-glucronide

<0.4

 

 

Other hormones

 

Compound

% Reactivity

E2 ELISA Kit

cis -Androsterone

<0.03

trans -Androsterone

<0.03

Cholesterol

0.46

Dehydroisoandrosterone

<0.03

5α-Dihydroteststerone

0.38

Hydrocortisone

0.38

Pregnenolone

0.35

Teststerone

<0.03

Kit Format

Kit Format

Comment

Microplate

1 plate (96 wells)& reagents

Needs a microplate reader (450nm)
For multiple and simultaneous measurement

 

 

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