E1 monoclonal antibody binds exclusively with E1 and does not show cross-reaction with other chemicals with similar structures.
The quantitative analysis ranges from 0.05μg/L- 5μg/L(ppb), sensitive enough to detect E1 in a specimen from river or waste water, etc.
With ease of handling, the total time for measurement is only 2.5 hours.
The ELISA measurement is highly reproducible; the coefficient of variation (CV) is generally under 10%.
The kit, a 96-well microplate format, enables simultaneous measurement of multiple samples at more reasonable cost.
Estrone (E1) is found more abundantly in river or sewage water than 17β-Estradiol (E2), thus requiring cautious monitoring, although estrogenicity of E1 is reported to be weaker than that of E2 in the vitellogenin synthesis test in rainbow trout.
E1 Standard Curve
Samples containing Estrone(E1) within the dynamic range (0.05 μg/L- 5 μg/L) can be directly applied to assay after filtration. Samples with Estrone content below the range must be concentrated with solid phase extraction prior to the ensuing session. Coefficient of variation(CV) is generally under 10% throughout the dynamic range.