本研究由上海第三人民医院方勇教授课题组完成，已经被Journal of Burn Care and
Hydrogen-Rich Saline Protects against Acute Lung Injury Induced by
Extensive Burn in Rat Model
Objectives Hydrogen has been reported to selectively quench detrimental
reactive oxygen species, particularly hydroxyl radical, and to prevent
myocardial or hepatic ischemia/reperfusion injury in multiple models. The aim of
this study is to investigate whether hydrogen protects against severe
burn-induced acute lung injury in rats.
Methods Rats were divided into four groups: sham plus normal saline, burn
injury plus normal saline, burn injury plus hydrogen-rich saline, and burn
injury plus Edaravone. Animals were given full-thickness burn wounds (30% total
body surface area) using boiling water, except the sham group which was treated
with room-temperature water. The rats in hydrogen group received 5 ml/kg of
hydrogen-rich saline, sham and burn controls obtained the same amount of saline,
and the Edaravone group was treated with 9 mg/kg of Edaravone in saline.
Lactated Ringer's solution was given at 6 h post-burn. Lungs were harvested at
12 h post-burn for laboratory investigations.
Results Severe burns with delayed resuscitation rapidly caused lung edema
and impaired oxygenation in rats. These dysfunctions were ameliorated by
administration of hydrogen-rich saline or Edaravone. Compared with the burn
injury plus normal saline group, hydrogen-rich saline or Edaravone significantly
attenuated the pulmonary oxidative products, such as malondialdehyde, carbonyl,
and 8-hydroxy-2'-deoxyguanosine. Furthermore, administration of hydrogen-rich
saline or Edaravone dramatically reduced the pulmonary levels of pulmonary
inflammation mediators and myeloperoxidase.
Conclusion Intraperitoneal (i.p.) administration of hydrogen-rich saline
improves pulmonary function by reducing oxidative stress and inflammatory
response in severe burn-induced acute lung injury.