在LTQ Orbitrap XL ETD上使用ETD和CID组合方法增强酪氨酸磷酸化蛋白质组鉴定覆盖率

简介：

A variety of strategies ranging from mass spectrometric (MS) based methods to phosphopeptide enrichment prior to MS have been developed to overcome the underrepresentation of tyrosine phosphorylation5, 6, 8. Immuno-affinity enrichment by antibodies directed against the tyrosine phosphorylated residue can be used for both tyrosine phosphorylated proteins and peptides. Currently, immuno-affinity purification (IP) of tyrosine phosphorylated peptides has demonstrated a high success rate for detection and site localization of tyrosine phosphorylation5-7. Despite recent advances, the tyrosine phosphoproteome is far from comprehensive and therefore ongoing method development is still essential. Here, we utilized the Thermo Scientific LTQ Orbitrap XL ETD to identify the site of phosphorylation from a peptide immuno-affinity purification of pervanadate treated HeLa cells.

仪器：

Thermo Scientific LTQ Orbitrap XL ETD