LC-MS/MS数据关联模式下使用0.5ppm质量精度的SIM鉴定
简介:Purpose: Combination of dynamic range enhancement andimprovement of mass accuracy for protein identification in complex mixtures
Methods: Nano-LC-MS and nano-LC-CID-MS/MS using a Hybrid Linear Ion Trap–FTICR Mass Spectrometer (Thermo Scientific LTQ FT)
Results: Demonstration of high dynamic range with high mass accuracy (<0.5ppm) which greatly improves the coverage and identification of proteins using a protein mixture.
仪器:Nano-LC-MS and nano-LC-CID-MS/MS
结论:
• The described method setup offers mass accuracy of better than 0.5 ppm for the precursor ion;
• Improved dynamic range by accumulating more ions (10-fold increase) for the FT master scan;
• Improved dynamic range results in more identification with higher sequence coverage;
• SIM scan in the ICR cell and the MS/MS experiment in the linear trap are performed in parallel;
• Shorter database search times due to excellent mass accuracy;
• The probability of false positive hits is greatly reduced.