使用高分辨三重四级杆质谱对复杂样品中的目标肽段进行定量
简介：In this study, we demonstrate the instrumental advantages afforded by the TSQ Quantum Ultra for highly selective and highly sensitive confident targeted peptide quantitation in whole human serum digests. A new approach for simultaneous targeted peptide identification and quantitation, based on high resolution precursor ion isolation and time alignment of multiple fragment ions from targeted peptides, is described.
This approach is also compared to the traditional SRMtriggered MS/MS approach for peptide quantitation, QED-MS/MS on a TSQ Quantum Ultra, and MRMtriggered Enhanced Product Ion Scan (EPI MS/MS) from a 4000 Q TRAP® (Applied Biosystems, Foster City, CA). The analytical precision of the H-SRM assay was evaluated vs. that of the SRM assay on the TSQ Quantum Ultra and on the 4000 Q TRAP using the same transitions and dwell times. Lastly, the quantitative accuracy of the H-SRM assay was evaluated.
结论：A new approach using the H-SRM assay on a TSQ Quantum Ultra triple quadrupole mass spectrometer for simultaneous qualitative and quantitative targeted protein analysis was evaluated. This approach gave excellent sensitivity, nalytical assay precision and quantitative accuracy for targeted protein quantitation in whole human serum by dramatically reducing non-specific interference from serum background ions. The H-SRM assay greatly improves assay specificity and detection limits thereby offering significant advantages for biomarker verification and validation studies.
