一种灵敏高效的人血清中差异表达的内源性肽段非标记定量分析方法
简介：
In this study, we describe the development of a workflow specifically geared toward the efficient recovery and identification of endogenous peptides from blood utilizing a combination of up-front batch sample prep and on-line liquid chromatography coupled with high-resolution tandem MS using HCD and CID fragmentation on an LTQ Orbitrap XL™. Quantitative differential analysis of the endogenous peptides was carried out using label-free analysis with SIEVE™ software algorithm. The novel workflow was applied to investigate the quantitative differences in endogenous peptides recovered from maternal serum in normal and Trisomy 21 (Down’s Syndrome) pregnancies.
仪器：
结论：
• The described workflow facilitates the recovery of endogenous peptides from blood.
• Endogenous peptides may constitute a viable source of disease biomarkers.
• The results of this study demonstrate that the majority (ca 90%) of endogenous peptides were up-regulated in the disease state. This result is consistent with the hypothesis that endogenous peptide fragments represent a “metabolic snapshot” of proteolytic activities induced by disease or inflammation. Therefore, only an increase and not a decrease in endogenous peptides would be expected in disease samples.
• In this study, we present preliminary data that demonstrate an increase in peptides associated with lipid metabolism detected in maternal serum from Trisomy 21 pregnancies.
