使用同位素标记内标和高分辨SRM质谱对目标内源性唾液肽段的绝对定量

简介：To develop a highly sensitive, selective, accurate and precise method for absolute quantitation of endogenous salivary peptides on a triple quadrupole mass spectrometer by using isotopically labeled peptides as internal standards.

仪器：TSQ Vantage

结论：

1. A 46-transitions H-SRM assay on a new TSQ Vantage triple quadrupole mass spectrometer was used to successfully determine the absolute amounts of six targeted endogenous peptides from parotid and sublingual/ submandibular gland secretions of eight human donors using the AQUA strategy without any prior peptide enrichment or sample clean up.

2. The H-SRM assay was highly sensitive and enabled to detect the native salivary peptides at less than 10 attomole/μL level.

3. The analytical precision of the H-SRM assay was good. The RSDs of five peptides were less than 12% (average 8.5%). The RSD of the low-level peptide AAPDEKVLDSGFR was also within acceptable range (25%).

4. Significant abundance variations for each targeted endogenous peptide were observed between donors and between glandular sources. The biological meaning for these variations is a subject of further investigation.