使用TMT和PQD对蛋白酶解物进行相对定量

　　简介：

To demonstrate the benefits of the PQD-based quantitation of isobarically labeled peptides in protein digests.

　　仪器：

　LTQ XL

　　结论：

• Optimization of PQD parameters for LC MS/MS analysis is important for reproducible and accurate protein quantitation.

• The PQD fragmentation technique produces high quality MS/MS spectra with good signal to noise for TMT reporter ions.

• The accuracy of the TMT ratios was better than 19% on the protein level.

• Proteome Discoverer performs protein identification and protein quantitation using the MS/MS spectra generated by PQD. Reporter ion intensities of the MS/MS spectra were successfully used for peptide and protein quantitation.