使用iSRM模式进行快速准确的目标蛋白质定量

　简介：Demonstrate the utility of iSRM technology by developing and refining an iSRM workflow for the simultaneous analysis of 40 proteins. Then, use the iSRM workflow to identify and quantify those proteins in a single analysis and ssess the precision of the resulting measurements.

　　仪器：TSQ Vantage

　　结论：The newly eveloped intelligent SRM (iSRM) technology provides the easiest and fastest way to develop the robust, accurate, and sensitive SRM assays for targeted protein quantification. A validated, highly complex iSRM assay to 　simultaneously quantify and identify 40 yeast proteins was developed in few hours by using Pinpoint software and the iSRM workflow.