使用台阶式高能碰撞解离提高TMT标记肽段定量鉴定效率
简介:The goal of the research reported in this application note was to investigate the effects of stepped HCD collision energy on relative quantitation of TMT-labeled peptides.
结论:
• A single-value, optimized collision energy can produce good identification and reporter (TMT)-based quantitation results for most peptides.
• For peptides that do not yield adequate quantitation results at a fixed CE, a stepped CE method can be used.
• Stepped CE showed overall improvement of quantitation results including the number of quantified peptides and reproducibility of quantitation.
• Increasing the number of microscans for a single-value, optimized collision energy improved the quality of spectra, but it did not significantly
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