使用LTQ FT对人血浆蛋白质组中的低分子量蛋白进行Top Down分析

　简介：Demonstrate the effectiveness of a specific enrichment methodology, along with top-down analysis on the LTQ FT, to detect and identify both intact LMW plasma proteins and peptides belonging to the plasma peptidome in an enzyme-free protocol.

仪器：LTQ FT

结论：The Low Molecular Weight portion of the human plasma proteome is thought to contain a wealth of putative iomarkers for human disease. This study was performed to demonstrate an effective methodology for isolating the LMW protein fraction of plasma and analyzing it directly with Top-Down protein sequence analysis. To be effective, Top-Down analysis requires high mass accuracy and resolution in order to determine the molecular weight of the intact protein and its fragments. To be practical, Top-Down analysis should be performed during routine LC/MS of LMW plasma protein fractions. As such, the LTQ FT is the only analytical system capable of performing such an analysis. Data is shown for just one of many proteins identified in this analysis. Further studies will demonstrate online multidimensional chromatography techniques to enhance the separation of the intact proteins for future differential expression work.