Nanospray ESI和离子阱多级质谱鉴定蛋白质的糖基化修饰

简介：

The accurate identification of glycopeptides is challenging because of their complex structures, labile nature, and microheterogeneity. A variety of mass spectrometry based techniques for determining the structure of glycopeptides have been used, including Time of Flight (TOF), triple quadrupole, and ion trap, coupled with a variety of ionization techniques including fast atom bombardment (FAB), matrix-assisted laser desorption ionization (MALDI), and electrospray ionization (ESI). This application report describes the use of a static nanospray ionization technique in combination with a novel linear ion trap (Thermo Scientific LTQ) for identification and characterization of glycosylation in complex mixtures.

质谱：

LTQ ion trap mass spectrometer

结论：

The LTQ ion trap mass spectrometer has the sensitivity and scan functionality for the analysis of glycoproteins. Using the unique MS/MS and MSn functions, combined with BioWorks protein identification software, the glycosylated peptide was identified and the sugar structure was determined. The MS4 spectra provided the additional information of the site of sugar attachment at the peptide backbone. The Advion NanoMate allows full automation of the nanospray process, resulting in excellent reproducibility while minimizing sample consumption and eliminating cross-contamination.