采用HPLC测定氯化两面针碱，toddalolactone，和白屈菜红碱

摘要：

Zanthoxylum nitidum (Roxb.) DC (Figure 1A) is an important traditional Chinese medicine. The Pharmacopeia of the People’s Republic of China (PPRC) 2010 regulates this dried root as an herbal medicine. Nitidine (structure of its chloride shown in Figure 2) is the specifi c active component in the dried root version of Zanthoxylum nitidum (Roxb.) DC, and nitidine chloride is reported to be benefi cial for both killing and constraining the growth of cancerous cells. For example, it may inhibit the proliferation of human hepatocarcinoma cell line SMMC-7721 and renal carcinoma cell line Ketr-3,3,4 induce the apoptosis of tumor cell lines SPC-A-1 and Tea8113, and decrease the cell survival probability of human nasopharyngeal cancer cell line CNE1 and breast cancer cell line MDA-MB-231. Zanthoxylum nitidum var. fastuosum (Figure 1B) is another plant in the same genus as Zanthoxylum nitidum (Roxb.) DC. Although Zanthoxylum nitidum var. fastuosum is not regulated in the PPRC, its dried root is still used in Chinese folk medicine because some of its reported medical benefi ts, such as promotion of blood circulation, pain relief, treatment of gastric ulcers, and reduction of infl ammation, are the same as those reported for Zanthoxylum nitidum (Roxb.) DC.The major active components of Zanthoxylum nitidum (Roxb.) DC and Zanthoxylum nitidum var. fastuosum are alkaloids (e.g., chelerythrine chloride, structure shown in Figure 2). Nitidine and toddalolactone (structures shown in Figure 2) are the specific active components in Zanthoxylum nitidum (Roxb.) DC and Zanthoxylum nitidum var. fastuosum, respectively.2 The PPRC 2010 method regulates the quality control of Zanthoxylum nitidum (Roxb.) DC using a high-performance liquid chromatography (HPLC)method for determination of nitidine chloride, but uses a thin-layer chromatography (TLC) method for detection of chelerythrine chloride and toddalolactone, where the presence of toddalolactone is not permitted.

仪器：

Thermo Scientific Dionex 优谱佳UHPLC+高效液相色谱系统

结论：

This work describes an HPLC method that uses UV absorbance detection to rapidly determine nitidine chloride, chelerythrine chloride, and toddalolactone in Zanthoxylum nitidum (Roxb.) DC and related products.The determination was performed on the UltiMate™ 3000 HPLC system controlled by Chromeleon™ CDS software. The Acclaim PA column delivers good peak shape for nitidine chloride and chelerythrine chloride, using an ammonium acetate buffer/acetonitrile mobile phase without triethylamine. This method can be used for quality control of Zanthoxylum nitidum (Roxb.) DC and related products. This approach is superior to both the PPRC method that measures the purported active components of Zanthoxylum nitidum (Roxb.) DC using two different methods—HPLC and TLC—and the HPLC method that requires a long separation time.