利用LC-MS/MS筛查尿液和血清中的药物和毒物

前言:

Screening for drugs of abuse and other toxic compounds in biological samples has quickly become a routine assay conducted in many research toxicology laboratories. The main challenge is to get rapid and accurate results amidst the generally large number of potential analytes to be identified within complex biological matrices. One of the techniques widely used in this area is high pressure liquid chromatography (HPLC) combined with photo diode array detection (DAD) or ultra-violet (UV) detection. The most popular LC-UV platform has been the Bio-Rad® REMEDi™ HS drug profiling system. When this platform was recently discontinued, a significant technological gap became apparent. Now this gap is rapidly being filled by newer, more effective high pressure liquid chromatography-mass spectrometry (HPLC-MS) technologies.

Here we present the workflow and results obtained by using the Thermo Scientific ToxSpec Analyzer, a new UHPLC-MS system based on ultra-high pressure liquid chromatography and linear ion trap mass spectrometry technology.

仪器:

LXQ™ linear ion trap mass spectromete

Accela™ UHPLC system

结论:

More than 150 real laboratory samples (serum and urine) have been analyzed. Table 1 reports some of the data obtained from both the REMEDi HS LC/UV system and the ToxSpec Analyzer UHPLC/MS system. Among the 12 samples reported here, 22 compounds have been identified using both the REMEDi HS and the ToxSpec Analyzer. Notably however, the ToxSpec Analyzer system identified 24 additional compounds that were not detected with the REMEDi HS due in most cases to a lack of sensitivity, specificity, and coelution capability.

The ToxSpec Analyzer also provided a better response for some classes of compounds, like benzodiazepines. With the REMEDi HS system, the retention time for this class of compounds was close to the dead volume of the column. For that reason, the signals that interfered with matrix components were rather difficult to identify. It was also observed that haloperidol (sample #5) and paroxetine (sample #10) gave a much better signal with the ToxSpec Analyzer.