使用三重四极杆质谱对母体药物进行代谢物鉴定及定量分析

前沿

Recent advances in the field of mass spectrometry have enhanced and expanded the capabilities of triple stage quadrupole (TSQ) mass spectrometers. These innovations include using linear ion traps to increase the product ion sensitivity; using high-Field Asymmetric waveform Ion Mobility Spectrometry (FAIMS) to increase the selectivity; and, more recently, using reverse energy ramp (RER) scanning to increase the product ion sensitivity. In addition, software developments, such as SRM-triggered data dependent scanning, have enabled the simultaneous acquisition of quantitative SRM data and qualitative product ion mass spectra on a TSQ instrument.

仪器：

Thermo Scientific TSQ Quantum Ultra™ triple stage quadrupole mass spectrometer

结论：

Quantitation-Enhanced Data-Dependent MS/MS scanning on a triple stage quadrupole mass spectrometer enables simultaneous metabolite identification and quantitation of a parent drug. The selectivity provided by this technique helps to increase the detection of metabolites. When the QED-MS/MS and RER techniques are coupled, the structure and concentration profiles of parent drugs and metabolites can be obtained simultaneously, thus increasing the bioanalytical throughput in drug discovery laboratories.