使用LC–FAIMS–H-SRM分析尿液中剂盐酸克仑特罗反兴奋剂

前言:

Clenbuterol is a beta-2 agonist drug with anabolic properties that is commonly used as a bronchodilator in veterinary medicine (Figure 1). Its use in humans has been banned in many countries, including the United States, because of serious cardiovascular and pulmonary side effects. The short-term effects of clenbuterol are similar to stimulant drugs like amphetamine or ephedrine and include increased heart rate, temperature, and blood pressure. Clenbuterol also increases lean muscle mass while reducing fat deposition. Some athletes and bodybuilders use the drug for these thermogenic and anticatabolic effects, and as a result clenbuterol has been banned by the World Anti-Doping Agency (WADA).

FAIMS and H-SRM work together to increase assay selectivity. In the interface between the ion source and the mass spectrometer, FAIMS selects which ions are allowed into the vacuum region. By applying alternating low and high electric fields, interferences are filtered out. The result is LC-MS/MS chromatograms with reduced chemical background and endogenous interferences. H-SRM, in turn, provides higher analyte selectivity through improved mass resolution of the precursor ion with Q1 while maintaining high transmission efficiency. The net result is cleaner chromatograms and more reliable results.

仪器:

TSQ Quantum Ultra™ triple stage quadrupole mass spectrometer

结论:

The combination of FAIMS and H-SRM provides excellent selectivity for the analysis of clenbuterol in human urine. Compared to the LC–SRM method, the use of LC–FAIMS–H-SRM reduced the chemical background and resulted in cleaner chromatograms and more reproducibly integrated chromatographic peaks. At the LLOQ, the clenbuterol assay in human urine shows the best accuracy and precision via LC–FAIMS–H-SRM.