Table of Contents
Index of Analytes........................................................................................................................................................................4
Introduction to Pharmaceuticals.................................................................................................................................................5
UltiMate 3000 UHPLC+ Systems.............................................................................................................................................6
IC and RFIC Systems.................................................................................................................................................................7
MS Instruments..........................................................................................................................................................................8
Chromeleon 7 Chromatography Data System Software...........................................................................................................9
Process Analytical Systems and Software...............................................................................................................................10
Automated Sample Preparation...............................................................................................................................................11
Analysis of Over-the-Counter Drugs...................................................................................................................................12
Accelerated USP Assay of Aspirin on the Acclaim 120 C18 RSLC Column.........................................................................13
Determination of Four Active Ingredients in a Multi-Symptom Cold Remedy Using Acclaim PA......................................14
Fast Analysis for Loratidine and Pseudo-Ephedrine in a Time-Release Tablet on Acclaim PA.............................................15
Miconazole in Athlete's Foot Ointment on Acclaim 120 C18.................................................................................................17
Ketoconazole in Anti-Dandruff Shampoo on the Acclaim 120 C18 Column.........................................................................18
Assay for Water-Soluble Vitamins...........................................................................................................................................20
Fat-Soluble Vitamins and Carotenoids in a Vitamin Tablet on Acclaim PA...........................................................................21
Analysis of APIs in a Pain-Relief Medicine on Acclaim Mixed-Mode WAX-1....................................................................23
Nonsteroidal Anti-Inflammatory Drugs on Acclaim PA2 C18................................................................................................24
Hydrocortisone in Skin Ointment............................................................................................................................................25
Omeprazole in Anti-Heartburn Tablet on the Acclaim PA2 Column......................................................................................27
Alkaloids in Bitter Orange on Acclaim 120 C18.....................................................................................................................29
Determination of Ten Active Ingredients in Sunscreen-Containing Products in a Single Injection......................................30
Isocratic Separation of Basic, Neutral, and Acidic Molecules on Acclaim Mixed-Mode WAX-1........................................36
Gradient Separation of Basic, Neutral, and Acidic Pharmaceuticals on Acclaim Mixed-Mode WAX-1..............................38
Determination of Aucubin, Genipoide, and Pinoresinol Diglucoside in Cortex Eucommiae Using ASE and HPLC...........39
Separation of Sinapine Thiocyanate in Semen Raphani Using an Acclaim Phenyl-1 Column.............................................41
Determination of Anthraquinones and Stilbenes in Giant Knotweed Rhizome by HPLC with UV Detection.....................43
Analysis of Benzalkonium Chloride on the Acclaim Surfactant Column
by High-Performance Liquid Chromatography...............................................................................................................50
Determination of Ethylene Glycol and Diethylene Glycol in a Sorbitol Solution.................................................................56
HPLC Assay of Water-Soluble Vitamins, Fat-Soluble Vitamins, and a Preservative
in Dry Syrup Multivitamin Formulation..........................................................................................................................66
Column Selection Guide........................................................................................................................................................80
Thermo Scientific Acclaim Column Selection Guide.............................................................................................................81
Transferring HPLC Methods to UHPLC............................................................................................................................82
Easy Method Transfer from HPLC to RSLC with the Dionex Method Speed-Up Calculator..............................................83