Table of Contents
Index of Analytes........................................................................................................................................................................3
Introduction to Pharmaceuticals.................................................................................................................................................4
UltiMate 3000 UHPLC+ Systems..............................................................................................................................................5
IC and RFIC Systems.................................................................................................................................................................6
MS Instruments..........................................................................................................................................................................7
Chromeleon 7 Chromatography Data System Software...........................................................................................................8
Process Analytical Systems and Software.................................................................................................................................9
Automated Sample Preparation...............................................................................................................................................10
Analysis of Antibiotics............................................................................................................................................................11
Determination of Tobramycin and Impurities Using HPAE-IPAD.........................................................................................12
Determination of Neomycin B and Impurities Using HPAE-IPAD........................................................................................24
Determination of Sulfur-Containing Antibiotics Using Integrated Pulsed Amperometric Detection (IPAD).......................38
Determination of Streptomycin and Impurities Using HPAE-PAD........................................................................................50
Analysis of Paromomycin by HPAE-IPAD.............................................................................................................................64
Determination of Sulfate Counter Ion and Aionic Impurities in Aminoglycoside Drug Substances
by Ion Chromatography with Suppressed Conductivity Detection.................................................................................72
Determination of N-Methylpyrrolidine in Cefepime Using a Reagent-Free Ion Chromatography System..........................82
Determination of Cefepime and Cefepime-Related Substances Using HPLC with UV Detection.......................................90
Sulfonamide Antibiotics on Acclaim 120 C18 RSLC.............................................................................................................98
Rapid HPLC Separation of Multiclass Antibiotics in Food and Water...................................................................................99
Determination of N-Methylpyrrolidine in Cefepime with Nonsuppressed Conductivity Detection...................................101
Determination of 2-Ethylhexanoic Acid Impurity in Clavulanate........................................................................................107
Analysis of the Aminoglycoside Antibiotics Kanamycin and Amikacin Matches USP Requirements...............................113
New HPLC and UHPLC Assay Methods for Tetracycline Hydrochloride and 4-Epianhydrotetracycline
Hydrochloride Impurity in Tetracycline Drug Products.................................................................................................119
Determination of Tobramycin in Crude and In-Process Production Samples During Manufacturing
Using HPAE-IPAD..........................................................................................................................................................126
Column Selection Guide......................................................................................................................................................132
Thermo Scientific Acclaim Column Selection Guide...........................................................................................................133
Transferring HPLC Methods to UHPLC..........................................................................................................................134
Easy Method Transfer from HPLC to RSLC with the Dionex Method Speed-Up Calculator............................................135