法医：用价格适中、高分辨率、精确质量的质谱定量尿中ng/mL级鸦片

Quantitation of Opiates to Low ng/mL Levels in Urine for Forensic Use Using an Affordable, High-Resolution, Accurate-Mass Mass Spectrometer

Key Words

Q Exactive Focus, opiates, morphine, codeine, hydromorphone, hydrocodone, oxymorphone, oxycodone, TraceFinder, forensic toxicology, drugs of abuse, PRM, parallel reaction monitoring

Goal

To evaluate the performance of the Thermo Scientific™ Q Exactive™ Focus hybrid quadrupole-Orbitrap™ mass spectrometer as a quantitative platform for HPLC-MS analysis of opiates in human urine to low ng/mL levels for forensic toxicology.

Introduction

Forensic toxicologists need an economical instrument capable of both screening a large number of compounds and quantifying smaller panels to industry-established limits. Here we present a method for quantitation of six opiates—morphine, codeine, hydromorphone, hydrocodone, oxymorphone, and oxycodone—in human urine down to low ng/mL levels. This work was performed on a Q Exactive Focus hybrid quadrupole-Orbitrap mass spectrometer.

Conclusion

The Q Exactive Focus MS accurately quantitated all six opiates tested to the low ng/mL level in human urine. This new instrument gives forensic laboratories a single versatile platform capable of both screening large panels1 and quantitative confirmation of specific panels that provides performance with value.