用CE-MS分析植物提取物中的黄酮类化合物

Abstract

Complex extracts of plant compounds often need a very effective separation mechanism for a clear compound identification. MS detection can provide a specific identification of the analyte in such complex and difficult matrices. Here we show the analysis of flavonoids from extracts of oranges, illustrating the specificity and sensitivity of an Electrospray Mass Spectrometer  (ESI-
MS) which can add a new dimension to capillary electrophoresis separations.

Hesperidine, a flavone glucoside, is present in the peel of green oranges while naringin, a rhamnoglucoside flavanone, is a bitter component of the orange peel. If not controlled properly, the high squeeze pressure applied to oranges during production of orange juice yields a high content of these flavonoids which gives it a bitter taste.  Monitoring the presence of these in orange juice provides a way of monitoring the quality of the product.

CE was the separation technique of choice to allow a fast and efficient separation and additionally to reduce sample preparation to centrifugation and filtering of the juice.