赛默飞拓展UHPLC技术用于生物制药的工作流程

Thermo Fisher Scientific Extends UHPLC Performance to Biopharmaceutical Workflows

Thermo Scientific Dionex UltiMate 3000 BioRS chromatography system combines corrosion resistance and UHPLC capability for bioseparations

【导读】"There is strong demand for a true high-performance biological separation platform, offering capabilities beyond reverse phase UHPLC. This combination of state-of-the-art technology and low-dispersion flow path is engineered to provide the high-peak capacity and sensitivity needed to analyze complex biomolecules." -See more at: http://news.thermofisher.com/press-release/thermo-scientific/thermo-fisher-scientific-extends-uhplc-performance-biopharmaceutical#sthash.OLm6LD1P.dpuf

“We’ve designed this system to accelerate research into promising biotherapies, such as monoclonal antibody variants, and also to support biopharmaceutical research and QA/QC, including glycan analysis,” said Wulff Niedner, director, HPLC product marketing, for Thermo Fisher Scientific. “There is strong demand for a true high-performance biological separation platform, offering capabilities beyond reverse phase UHPLC. This combination of state-of-the-art technology and low-dispersion flow path is engineered to provide the high-peak capacity and sensitivity needed to analyze complex biomolecules.”

The system was developed for reliability and durability, even with harsh solvents used for many bioseparations. The flow path is built of corrosion-resistant materials, enhancing robustness and extending the life of biocolumns, even under high salt or extreme pH conditions. In addition, the materials used are designed to significantly reduce the risk of analytes interacting with internal surfaces of the flow path, protecting data quality.

The UltiMate 3000 BioRS system is powered by UltiMate 3000 Rapid Separation technology to support the high pressures required to separate many bioanalytes on high resolution UHPLC columns. The system is engineered to operate at pressures as high as 1,000 bar (15,000 psi) and flow rates up to 8 mL/min. This wide flow/pressure footprint supports many biomolecule characterizations, including development, identification and quality control. The tool-free biocompatible Thermo Scientific Dionex Viper fingertight fitting system is designed to facilitate connections without dead volume to combat peak-broadening effects.

The modular system offers many configuration options to accommodate most large molecule workflows. These include binary, quaternary and dual gradient mobile phase delivery, as well as parallel and tandem LC configurations.

Columns designed to optimize system performance

Thermo Fisher Scientific also chose Pittcon 2013 to introduce columns developed specifically to complement the performance of the UltiMate 3000 BioRS system. The Thermo Scientific MAbPac SCX-10 RS column is designed for UHPLC separations of monoclonal antibodies. The Thermo Scientific DNAPac PA200 RS column can be used to analyze single nucleic acids and isomers to yield important structural information.

Both feature PEEK linings, providing highly inert flow paths designed to remain stable at UHPLC pressures, and ultra-stable packed beds designed for excellent retention reproducibility and long life.

Another biocolumn choice is the new Thermo Scientific Dionex GlycanPac AXH-1 column, designed to enhance glycan analysis with simultaneous charge, size and polarity HPLC separations.

For more information about the Thermo Scientific UltiMate 3000 BioRS UHPLC system, visit www.thermoscientific.com/BioRS.

About Thermo Fisher Scientific

Thermo Fisher Scientific Inc. is the world leader in serving science. Our mission is to enable our customers to make the world healthier, cleaner and safer. With revenues of $13 billion, we have approximately 39,000 employees and serve customers within pharmaceutical and biotech companies, hospitals and clinical diagnostic labs, universities, research institutions and government agencies, as well as in environmental and process control industries. We create value for our key stakeholders through three premier brands, Thermo Scientific, Fisher Scientific and Unity™ Lab Services, which offer a unique combination of innovative technologies, convenient purchasing options and a single solution for laboratory operations management. Our products and services help our customers solve complex analytical challenges, improve patient diagnostics and increase laboratory productivity. Visit http://www.thermofisher.com.

-See more at: http://news.thermofisher.com/press-release/thermo-scientific/thermo-fisher-scientific-extends-uhplc-performance-biopharmaceutical#sthash.OLm6LD1P.dpuf