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工商注册信息已核实!领域: | 蛋白/抗体/蛋白质组,其他生命科学 | ||
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High-speed MALDI-TOF/TOF imaging of mouse brain tissue performed on intact proteins and after on-tissue digestion |
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The unique flexibility of MALDI-TOF/TOF systems facilitates tissue imaging of intact proteins as well as of tryptic peptides resulting from on-tissue digestion. While intact protein imaging can provide information on the diversity of proteoforms, the on-tissue digestion approach benefits from significantly higher mass resolution and accuracy that can be achieved for tryptic peptides in reflector operation mode as well as from additional specificity provided by MS/MS analysis.