单分子定位超高分辨成像技术(Single Molecular Localization Super-resolution)已问世十年有余,逐渐成为生命科学研究中的常规实验手段。Bruker的Vutura产品采用双焦面三维实时单分子定位技术,对样品的两个焦面同时成像,一次成像(两张图片)就可以得到单分子荧光信号三维位置信息。
另外,成像速度快,光毒性小。横向分辨率20nm,轴向分辨率50nm,成像深度可达15μm。并且支持超高分辨与高速共聚焦融合成像,是能够进行活组织/细胞动态超高分辨成像的利器。
A Complete Solution for Super Resolution at the Nanoscopic Level
Enter the world of nanoscale imaging with the Vutara 352 super resolution microscope. Based on single molecule localization techniques (PALM, STORM, etc.), Vutara 352 enables quantitative imaging at the nanoscale.
Vutara’s proprietary Biplane technology provides 3-D imaging during all acquisitions.
A flat homogenous field of view using wide field illumination allows accurate imaging at depths of over 30 um.
A high-speed detection system and powerful lasers enable live cell nanoscale imaging.
Sophisticated visualization tools bring your results to life, and the Quantitative Localization Microscope analysis suite puts information at your fingertips.
Standard Acquisition Features
3-D localization data up to 1 um in Z for all acquisition
Video rate live cell nanoscale imaging
Z step acquisitions for thicker volumes
Real time localization during acquisition
Multiple location acquisition
Active Z drift correction
Simultaneous 2 color 3-D acquisition
Sequential and interleaved multicolor acquisition
Wide field reference images for localization experiments
Wide field work for wide field time lapse
Instrument options
Confocal scanner for correlative imaging
750 nm imaging laser
TIRF illumination
Analysis Features
Spatial distribution: Provides a variety of tools for analyzing spatial distribution relationships of particles including Ripley’s K, Pair correlation and Nearest Neighbors
Cluster Analysis: Counts clusters, cluster sizes, cluster densities, intra/inter-cluster relationships
Co-localization: Provides statistical measures on relationships between particles or clusters of two different labels
Resolution Analysis: Quantifies resolution for images derived from localized data sets
Live Cell Analysis: Tracking of clusters in live cell experiments with mean squared displacement and angular displacement analysis
Control Data: Generate statistically random data to use as comparative data controls
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