SpectraMax 190 是 Molecular Devices 推出一款具有超高性价比的全波长光吸收型酶标仪,专门针对96孔型微孔板优化检测光路,其内置高精度光栅型单色器,190nm 至 850nm 之间任意选择,2nm 最小带宽保证检测检测的高精度;高精度温度控制功能,控温范围室温 +4°C 至 45°C、也可进行线性震荡。
SpectraMax 190 采用多通道检测模式,可以像双波长分光光度计一样工作,由8个独立样品检测器和参照检测器组成,即减少一切由于光纤造成的光路输出误差,又可以增加检测速度;非温度依赖 pathcheck 光径传感器技术,可以将实测微孔板中检测到的吸光度值自动校正为 1cm 比色皿光径下的吸光度值,使对微孔板的测读达到分光光度计的精度,校正结果不随温度变化而变化,专业级别 SoftMax Pro 软件可自动进行数据的运算及存储,可完成图表曲线制作,19种曲线拟合方式,用户可自行编辑公式和程序。
主要特点
UV/可见光区 190-850nm,1nm 步进
单孔最高可检测6个波长
终点法、动力学法、光谱扫描法
Pathcheck 光路校正功能
精确的温度控制和自动震荡功能
SpectraDrop 超微量检测模块,仅需2uL,最高通量64个样品/次,dsDNA 检测灵敏度 2ng/uL
支持 StakMax 全自动堆板机
应用领域
主要可针对 DNA、RNA 和蛋白质的快速定量检测,广泛应用于 ELISA/EIA 检测、微生物生长/MIC、IC50/LD50、细胞毒性/细胞增殖、细菌内毒素检测、报告基因检测、细胞因子检测,且具有动力学检测模式,也可以进行酶学检测和动力学 ELISA 检测等。
实验举例
SpectraMax 190 采用 PathCheck 传感器,是一种校正样品液面高度(光径)的先进zl技术,且校正结果不受到温度变化影响,它能自动把微孔板中的样品高度转化为1cm的比色杯光径,可以消除微孔板内各液面的差异、扩大动态分析的范围、检测加液错误等。使用全程光谱扫描模式,您可以对样品的光谱检测进行全程监控,并选择最理想的波长去进行读测。
引用次数*:8,720
Anti-oxidative and hepatoprotective effects of lithospermic acid against carbon tetrachloride-induced liver oxidative damage in vitro and in vivo
KWK Chan, WS Ho - Oncology reports, 2015 - spandidos-publications.com
... The plate was gently shaken, and the absorbance was measured at 540 nm on a microtiter plate
reader (SpectraMax 190; Molecular Devices). LDH assay. ... The absor- bance at 490 nm was
measured by a microtiter plate reader (SpectraMax 190; Molecular Devices). ...
[HTML] Intravaginal Lactic Acid Bacteria Modulated Local and Systemic Immune Responses and Lowered the Incidence of Uterine Infections in Periparturient Dairy …
Q Deng, JF Odhiambo, U Farooq, T Lam, SM Dunn… - 2015 - dx.plos.org
... tetramethylbenzidine (TMB). After adding the stop solution, the plate was read at
450 nm on a microplate spectrophotometer (Spectramax 190, Molecular Devices
Corporation, Sunnyvale, CA) within 10 min. The optical density ...
Measurement of total iron in Helicobacter pylori-infected gastric epithelial cells
SE Flores, AS Day, JI Keenan - BioMetals, 2015 - Springer
... H. pylori were resuspended in RPMI-1640 medium supplemented with 10 % (v/v) heat-inactivated
fetal bovine serum and bacterial numbers were quantitated by density, based on a standard curve
measured at an absorbance of 620 nm (SpectraMax ® 190, Molecular devices ...
[HTML] The anti-inflammatory and antioxidant activity of 25 plant species used traditionally to treat pain in southern African
SA Adebayo, JP Dzoyem, LJ Shai… - … and alternative medicine, 2015 - biomedcentral.com
... After incubation, the absorbance was read at 450 nm in a microplate reader (SpectraMax 190,
Molecular devices). Quercetin (10 mM) was used as a standard. ... The absorbance of the reaction
mixture was recorded at 593 nm (SpectraMax 190, Molecular devices) after 5 min. ...
DCLAK11, a multi-tyrosine kinase inhibitor, exhibits potent antitumor and antiangiogenic activity in vitro
X Guo, X Chen, L Tong, X Peng, M Huang… - Acta Pharmacologica …, 2015 - nature.com
... The reaction was terminated by the addition of 50 μL of 2 mol/LH 2 SO 4 as the color
changed, and the absorbance at 490 nm was analyzed using a SpectraMax 190
Microplate Reader (Molecular Devices, Sunnyvale, CA, USA). ...
Digestive enzyme activities are higher in the shortfin mako shark, Isurus oxyrinchus, than in ectothermic sharks as a result of visceral endothermy
KC Newton, J Wraith, KA Dickson - Fish physiology and biochemistry, 2015 - Springer
... well microplate to measure the change in absorbance of the sample (relative to a negative control
using 50 mM Tris–HCl homogenization buffer substituted for tissue supernatant) in a
SPECTRAmax 190 microplate spectrophotometer (Molecular Devices, Sunnyvale, CA, USA). ...
Superparamagnetic iron oxide nanoparticles exacerbate the risks of reactive oxygen species-mediated external stresses
C Luo, Y Li, L Yang, X Wang, J Long, J Liu - Archives of toxicology, 2015 - Springer
... spectrophotometer (SPECTRAmax 190, Molecular Devices Corporation, USA). ... C0016,
Beyotime Biotechnology) and was recorded at 490 nm using a microplate spectrophotom-
eter (SPECTRAmax 190, Molecular Devices Corporation, USA). ...
[HTML] BMI1 Is Expressed in Canine Osteosarcoma and Contributes to Cell Growth and Chemotherapy Resistance
MH Shahi, D York, R Gandour-Edwards, SS Withers… - PloS one, 2015 - dx.plos.org
... 2hrs. Absorbance at 490nm was measured with a spectrophotometer (Spectramax
190, Molecular Devices LLC). Each group contained 6 wells and mean absorbance
is expressed as a percentage of untreated control cells. ...
[HTML] Effects of Benzo [a] pyrene Exposure on Human Hepatocellular Carcinoma Cell Angiogenesis, Metastasis, and NF-κB Signaling
Q Ba, J Li, C Huang, H Qiu, J Li, R Chu… - Environmental health …, 2015 - ncbi.nlm.nih.gov
... The attached cells were incubated with CCK-8 in medium for 4 hr and quantified
by measuring the absorbance at 450 nm with a SpectraMax 190 microplate reader
(Molecular Devices, Sunnyvale, CA, USA). Animal husbandry. ...
Activation of endoplasmic reticulum stress is involved in the activity of icariin against human lung adenocarcinoma cells
S Di, C Fan, Y Yang, S Jiang, M Liang, G Wu, B Wang… - Apoptosis, 2015 - Springer
... the plates were incubated at 37 °C in a 5 % CO 2 /95 % air humidified incubator for 2 h. Then,
optical density (OD) values were assessed at 450 nm using a microplate reader (SpectraMax
190, Molecular Devices, USA), and the cell viability was expressed as the OD value [ 30 ]. ...
* as of June 26, 2015. Source: Google Scholar
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