Waters HPLC故障排除指南
Waters公司的，HPLC故障排除指南
1. Column Life-Time
2. Variable Retention Times
3. Drifting Retention Times
4. Column-to-Column and Batch-to-Batch Reproducibility
5. Sample Preparation Problems
6. Sources of Peak Tailing
7. Normal-Phase Chromatography
8. System Volume, Dead Volume, Dwell Volume
9. Transfer of Gradient Methods
10. Clogged System
11. Column Plate-Count
12. Column Backpressure
13. Peak Area Fluctuations
14. Ghost Peaks
15. Dependence of Retention Times on pH
16. Column Equilibration
17. Column Conditioning
18. Complex Sample Matrices
19. Hydrophobic Collapse
20. Baseline Noise
21. Narrow-bore Columns
22. Sample Solvent
23. Gradient Scaling
24. Column Storage
25. Paired-Ion Chromatography
26. Hydrolytic Stability of Reversed-Phase
27. Optimal Flow Rates
28. Carbon Load
29. pH Control
30. Mobile Phase Composition
31. Column Contamination
32. Method Verification
33. Double Peaks in Sugar Separations
34. Method Control
35. Mobile Phase pH
36. Signal-to-Noise Improvements
37. Overload
38. Column Durability
39. Fast Analysis and Column Backpressure
40. Column Backflushing
41. Selectivity Shift
42. New Method
43. Negative Peaks
44. Tricky, Tedious, Time Consuming
45. Fast Separations
46. Buffers for LC/MS
47. Alkaline Buffers for RPLC
48. Post-Column Derivatization
49. Gradient Dwell Volume
50. Buffer Capacity
51. Flow Rate Changes and Quantitation
52. Analysis of polar compounds
