2010全国质谱大会大会报告（二）
美国纽约Mount Sinai 医学院 Wang, Rong（王融）教授
来自美国纽约Mount Sinai 医学院的Wang, Rong（王融）教授，做了题为“Fragmentation of Large Peptides Under Different Dissociation Conditions（在不同解离条件下碎裂大分子量多肽）”的报告。在报告中，王教授首先介绍了该研究的意义，了解大分子量多肽在不同碎裂条件下的碎裂性质和行为是基础的一项工作，更重要的是，在大分子量多肽分析中研究内源性生物技术，上述研究是必要的和具有实践意义的。课题组发展了一种系统的方法，利用化学合成的肽序列，使用不同的通用碎裂技术，以及HCD高能裂解技术，来比较多肽碎裂的效率、模式和氨基酸组成、多肽长度、电荷态、离子化方式（ESI或MALDI）之间的关系，并报告了课题组发现的结果。以下是英文摘要：
It is fundamental to understand fragmentation property and behavior of large peptides under different fragmentation conditions. More importantly, it is essential and practical in study endogenous biological techniques in analysis of large peptides. We conducted a systematical investigation of large peptide fragmentation using a chemically synthesized peptide ladders with several commonly used fragmentation techniques, and high energy collision induced dissociation. Peptide fragmentation efficiency and pattern were tested against amino acid composition, peptide length, and charge stage, as well as different ionization methods, e.g. electrospray ionization and matrix assistant laser desorption ionization. Detailed experimental findings will be reported.
中国地质科学院 刘敦一研究员
来自中国地质科学院的刘敦一研究员做了“阿波罗-12月岩锆石SHRIMP（高分辨二次离子质谱）U-Pb年龄测定”的报告。报告中，刘老师介绍了SHRIMP研究月岩锆石，获得了许多具有重要意义的数据。刘老师指出，月球同位素年代学研究复杂，难度很大，锆石SHRIMP定年是月岩定年的最佳手段，可用于原位微区分析，无需化学流程且对样品没有损伤。
美国PerkinElmer公司 Shen, Shida（沈世达）高级研究员
来自美国PerkinElmer公司的Shen, Shida（沈世达）高级研究员，做了题为“A High Resolving Power and Mass Accuracy Time-Of-Flight MS for Rapid Detection and Identification of Microorganisms”（用高分辨率高质量精度的飞行时间质谱快速测定微生物）的报告。在报告中，沈研究员介绍了公司研发的具有精确质量测定的ESI TOF质谱，可快速鉴定病原体和进行品系分类。这种独特的方法可以每个样品30秒的速度鉴定微生物，在针对生物战攻击或新传染病自然爆发时具有潜力。该方法使用了一种高端、低成本的ESI TOF质谱，报告介绍了该质谱仪在精确质量测定、高分辨和24小时稳定性方面的性能，并用猪流感病毒H1N1作为实例，阐述了检测和鉴定微生物的步骤。以下是英文摘要：
An exact mass measurement ESI TOF mass spectrometer has been developed for fast pathogen identification and strain typing. This unique technology relies on mass spectrometry-derived base composition signatures of amplicons from PCR (Polymerase Chain Reaction) products, and “intelligent PCR primers” that target broadly conserved regions of microbial genomes that flank variable regions. This rapid detection (30 seconds per sample) and identification of microorganism methodology also associates with a potential biological warfare attack or a natural outbreak of an emerging infectious disease.
The strategy to build a high end and low cost ESI TOF machine will be discussed. The performance data particularly in accurate mass measurement, high resolving power and 24 hours stability test will be presented. The discovery of Swine flu virus H1N1 will be presented as an example to illustrate the procedure of detection and identification of microoganisims.
