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王志勇教授最新文章解析重要激素调控机制

2007.9.05

来自美国卡内基研究院(Carnegie Institution),中科院植物研究所光合作用与环境分子生理学重点实验室(Key Laboratory of Photosynthesis and Environmental Molecular Physiology),加州大学旧金山分校,河北师范大学,斯坦福大学等处的研究人员提出磷酸化BR转录因子的有效抑制需要14-3-3蛋白的结合,从而证明了BR对于基因表达和职务生长的调控需要复合性机制。这一研究成果公布在《Developmental Cell》杂志上。

文章的通讯作者是来自卡内基研究院的王志勇教授,其早年毕业于兰州大学,主要从事植物激素信号传导研究,参予研究的还有植物研究所白明义等人。

同时他们也在《PNAS》杂志上发表了相关研究成果,他们通过RNAi技术对OsBZR1和14-3-3蛋白进行研究,找到一种新的调控OsBZR1活性的机制。这些机制的了解可使人们通过基因工程的方法精细调控水稻体内的油菜素内酯响应,为水稻高产育种提供重要的理论依据和新的操作手段。

原文检索:
Developmental Cell, Vol 13, 177-189, 07 August 2007
An Essential Role for 14-3-3 Proteins in Brassinosteroid Signal Transduction in Arabidopsis
[Abstract]

1970年,美国的米切尔(Mitchell)等报道在油菜的花粉中发现了一种新的生长物质,它能引起菜豆幼苗节间伸长、弯曲、裂开等异常生长反应,并将其命名为油菜素(brassin)。格罗夫(Grove)等(1979)用227kg油菜花粉提取得到10mg的高活性结晶物,因是甾醇内酯化合物而将其命名为油菜素内酯(brassinolide,BR1)。此后油菜素内酯及多种结构相似的化合物纷纷从多种植物中被分离鉴定,这些以甾醇为基本结构的具有生物活性的天然产物统称为油菜素甾体类化合物(brassinosteroids,BRs),BR在植物体内含量极少,但生理活性很强。

BR在植物界中普遍存在。油菜花粉是BR1的丰富来源,但其含量极低,只有100~200μg·kg-1,BR1也存在于其他植物中。BR2在已分析的植物中分布最广。BR主要用于增加农作物产量,减轻环境胁迫,有些也可用于插枝生根和花卉保鲜。随着对BR研究的深入和更有效而成本更低的人工合成类似物的出现,BR在农业生产上的应用必将越来越广泛,一些科学家已提议将油菜素甾醇类列为植物的第六类激素。

BR可以调控植物生长,这主要是通过诱导两个关键转录因子:BZR1和BZR2/BES1的去磷酸化来调控基因表达,这个信号传导途径包含了细胞表面受体BRI1和BAK1,以及一个GSK3激酶BIN2。但是至今BR如何调控磷酸化BZR1/BZR2活性的并不清楚。

在这篇文章中,研究人员发现BZR1/BZR2经BIN2催化的磷酸化不仅抑制了DNA的结合,而且促使了DNA与14-3-3蛋白的结合。BZR1上一个BIN2磷酸化位点的突变会扰乱14-3-3结合,从而导致BZR1蛋白核定位增强,加强了转基因植物中BR应答。而且BR缺陷也增加了细胞质定位,施用BR可以诱导BZR1/BZR2的快速核定位。因此研究人员认为14-3-3结合对于磷酸化BR转录因子的有效抑制是必需的,从而这一研究证明了BR对于基因表达和职务生长的调控需要复合性机制。

附:
王志勇,男,1964年10月出生,内蒙古人,博士,研究员。

电子邮件:zywang@andrew2.stanford.edu

电话:010-62836209

传真:010-82594821

研究方向: 植物激素与信号转导

Education:

1992-1998.1
Ph.D. in Molecular, Cell and Developmental Biology, University of California, Los Angeles.

1986-1989.6
M. S., Institute of Botany, Chinese Academy of Sciences, Beijing, China.

1982-1986.6
B. S., Department of Biology, Lanzhou University, Lanzhou, China.


Research experience:

Oct.2002-
Professor, Research Center for Molecular and Developmental Biology, the Institute of Botany, CAS.

June 2001-
Staff Member, Department of Plant Biology, the Carnegie Institution of Washington, Stanford. Plant molecular genetics, proteomics, hormone signaling, and development.

1998- 2001
Postdoctoral Research. The Salk Institute and the Howard Hughes Medical Institute, San Diego.

Molecular and genetic dissection of the brassinosteroid signal transduction pathways in Arabidopsis.

Advisor: Dr. Joanne Chory.

1992-1998
Ph. D. Thesis Research. Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles.

Functions of the Transcription Factor CCA1 in Circadian Rhythm and Light Regulation of Plant Gene Expression and Development

Advisor: Dr. Elaine M. Tobin

1991-1992
Research Assistant, Department of Biological Sciences, the State University of New York at Albany.

Molecular analysis of pollen specific gene expression and microspore development.

Advisor: Dr. Joseph Mascarenhas

1989-1991
Research Associate, National Lab of Plant Systemetics and Evolution, Chinese Academy of Sciences, Beijing, China.

Comparison of the pollen protein composition among families of gymnosperms using gel electrophoresis

Supervisor: Dr. Fu-Hsuing Wang

1986-1989
M.S. Thesis Research, The Institute of Botany, Chinese Academy of Sciences, Beijing, China

Biochemical and cell biological analysis of self-incompatibility of pollen-stigma interactions in Brassica pekinensis.

Advisors: Dr. Fu-Hsuing Wang and Prof. Peihua Tang

1985-1986
Undergraduate Research, Lanzhou University, China.

Changes of iso-peroxidase, iso-catalase activities and nucleic acid content during differentiation and de-differentiation of tobacco cells in tissue culture.

Advisor: Prof. Houguo Liang


GRANTS, FELLOWSHIPS and HONORS:

2002-2007 NIH R01 research grant.

1998-2000 Postdoctoral Fellowship in Bioscience Related to the Environment,

National Science Foundation.

1998 Postdoctoral Fellowship, the Howard Hughes Medical Institute.

1994 Pauley fellowship, UCLA.

1992  Award for Research Accomplishments in Science and Technology, Chinese Academy of Sciences, China; second level, second author.

1987  All-best Graduate Student Award, the Graduate School of the Chinese Academy of Sciences.

1983-1986 All-best Student Awards, Lanzhou University, Lanzhou, China.


PATENTS.

2002 A Gene for brassinosteroid regulation of plant growth and development

2002 A Cytochrome P450 involved in brassinosteroid biosynthesis.

1998 A transcription factor involved in phytochrome and circadian clock regulation of plant development.

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