13.080.30 土壤的生物特性 标准查询与下载

EN ISO 11267:2023 土壤质量 土壤污染物对跳虫（Folsomia candida）繁殖的抑制（ISO 11267:2023）

This document specifies one of the methods for evaluating the habitat function of soils and determining effects of soil contaminants and substances on the reproduction of Folsomia candida Willem by dermal and alimentary uptake. This document also provides information on how to use this method for testing substances under temperate conditions. The chronic test described is applicable to soils and soil materials of unknown quality, e.g. from contaminated sites, amended soils, soils after remediation, industrial, agricultural or other sites of concern and waste materials. The method is not applicable to volatile substances, i.e. substances for which H (Henry's constant) or the air/water partition coefficient is greater than 1, or for which the vapour pressure exceeds 300 Pa at 25 °C.

Soil quality - Inhibition of reproduction of Collembola (Folsomia candida) by soil contaminants (ISO 11267:2023)

ISO 11267:2023 土壤质量——土壤污染物对弹尾虫繁殖的抑制作用

Soil quality — Inhibition of reproduction of Collembola (Folsomia candida) by soil contaminants

23/30464262 DC BS ISO 17126 土壤质量 确定污染物对土壤菌群的影响 生菜幼苗（Lactuca sativa L.）出苗筛选试验

BS ISO 17126. Soil quality. Determination of the effects of pollutants on soil flora. Screening test for emergence of lettuce seedlings (Lactuca sativa L.)

T/GXAS 523-2023 污泥 粪大肠菌群的测定 酶底物法

本文件描述了使用酶底物法测定污泥中粪大肠菌群的方法。  本文件适用于城镇污水处理厂污泥、排水管道通挖污泥、污泥衍生产品中粪大肠菌群的测定。土壤和沉积物中粪大肠菌群的测定可参照本法。 本方法检出限为10 MPN/g。

Sludge—Determination of fecal coliforms—Enzyme substrate method

ISO/DIS 17126:2023 土壤质量 污染物对土壤菌群影响的测定 生菜幼苗（Lactuca sativa L.）出苗筛选试验

Soil quality — Determination of the effects of pollutants on soil flora — Screening test for emergence of lettuce seedlings (Lactuca sativa L.)

ISO/DIS 18187:2023 土壤质量 利用球形节杆菌脱氢酶活性对固体样品进行接触测试

Soil quality — Contact test for solid samples using the dehydrogenase activity of Arthrobacter globiformis

ISO/DIS 8259:2023 土壤质量 受污染土壤和类土物质中有机和无机污染物的生物可及性

Soil quality — Bioaccessibility of organic and inorganic pollutants from contaminated soil and soil-like material

BS EN ISO 16387:2023 土壤质量 污染物对Enchytraeidae(Enchytraeus sp.)的影响 对生殖影响的测定

1   Scope This document specifies one of the methods for evaluating the habitat function of soils and determining effects of soil contaminants and substances on the reproduction of Enchytraeus sp. by dermal and alimentary uptake in a chronic test. It is applicable to soils and soil materials of unknown quality, for example, from contaminated sites, amended soils, soils after remediation, agricultural or other sites under concern and waste materials. This document provides information on how to use this method for testing substances under temperate conditions. The method is not applicable to substances, for which the air/soil partition coefficient is greater than 1, or to substances for which the vapour pressure exceeds 300 Pa at 25 °C. NOTE No provision is made in the test method for monitoring the persistence of the substance under test.

Soil quality. Effects of contaminants on Enchytraeidae (Enchytraeus sp.). Determination of effects on reproduction

EN ISO 16387:2023 土壤质量.污染物对线蚓科的影响.对繁殖影响的测定

This document specifies one of the methods for evaluating the habitat function of soils and determining effects of soil contaminants and substances on the reproduction of Enchytraeus sp. by dermal and alimentary uptake in a chronic test. It is applicable to soils and soil materials of unknown quality, for example, from contaminated sites, amended soils, soils after remediation, agricultural or other sites under concern and waste materials. This document provides information on how to use this method for testing substances under temperate conditions. The method is not applicable to substances, for which the air/soil partition coefficient is greater than 1, or to substances for which the vapour pressure exceeds 300 Pa at 25 °C. NOTE No provision is made in the test method for monitoring the persistence of the substance under test.

Soil quality - Effects of contaminants on Enchytraeidae (Enchytraeus sp.) - Determination of effects on reproduction (ISO 16387:2023)

ISO 16387:2023 土壤质量.污染物对线蚓科的影响.对繁殖和存活率影响的测定

This document specifies one of the methods for evaluating the habitat function of soils and determining effects of soil contaminants and substances on the reproduction of Enchytraeus sp. by dermal and alimentary uptake in a chronic test. It is applicable to soils and soil materials of unknown quality, for example, from contaminated sites, amended soils, soils after remediation, agricultural or other sites under concern and waste materials. This document provides information on how to use this method for testing substances under temperate conditions. The method is not applicable to substances, for which the air/soil partition coefficient is greater than 1, or to substances for which the vapour pressure exceeds 300 Pa at 25 °C. NOTE No provision is made in the test method for monitoring the persistence of the substance under test.

Soil quality — Effects of contaminants on Enchytraeidae (Enchytraeus sp.) — Determination of effects on reproduction

BS EN ISO 11268-2:2023 土壤质量 污染物对蚯蚓的影响 确定赤子爱胜蚓/安氏爱胜蚓和其他蚯蚓物种的繁殖影响

1   Scope This document specifies one of the methods for evaluating the habitat function of soils and determining the effects of soil contaminants and chemicals on the reproduction of Eisenia fetida/Eisenia andrei by dermal and alimentary uptake. This chronic test is applicable to soils and soil materials of unknown quality, e.g. from contaminated sites, amended soils, soils after remediation, agricultural or other sites concerned, and waste materials. This method is designed mainly for determining the effects of soil contaminants and chemicals on the reproduction of Eisenia fetida/Eisenia andrei . Technical information is also provided on how to use Eisenia fetida/andrei for testing chemicals under tropical conditions (see Annex A). Finally, this method also includes technical information on how to use it with other environmentally relevant earthworm species: e.g. Dendrodrilus rubidus and Aporrectodea caliginosa (see Annexes B and C). This method does not apply to substances for which the air/soil partition coefficient is greater than one, or to substances with vapour pressure exceeding 300 Pa, at 25 °C. This method does not take into account the persistence of the substance during the test.

Soil quality. Effects of pollutants on earthworms. Determination of effects on reproduction of Eisenia fetida/Eisenia andrei and other earthworm species

EN ISO 11268-2:2023 土壤质量.污染物对蚯蚓的影响.第2部分:对赤子爱胜蚓/赤子安德列繁殖影响的测定

This document specifies one of the methods for evaluating the habitat function of soils and determining the effects of soil contaminants and chemicals on the reproduction of Eisenia fetida/Eisenia andrei by dermal and alimentary uptake. This chronic test is applicable to soils and soil materials of unknown quality, e.g. from contaminated sites, amended soils, soils after remediation, agricultural or other sites concerned, and waste materials. This method is designed mainly for determining the effects of soil contaminants and chemicals on the reproduction of Eisenia fetida/Eisenia andrei. Technical information is also provided on how to use Eisenia fetida/andrei for testing chemicals under tropical conditions (see Annex A). Finally, this method also includes technical information on how to use it with other environmentally relevant earthworm species: e.g. Dendrodrilus rubidus and Aporrectodea caliginosa (see Annexes B and C). This method does not apply to substances for which the air/soil partition coefficient is greater than one, or to substances with vapour pressure exceeding 300 Pa, at 25 °C. This method does not take into account the persistence of the substance during the test.

Soil quality - Effects of pollutants on earthworms - Part 2: Determination of effects on reproduction of Eisenia fetida/Eisenia andrei and other earthworm species (ISO 11268-2:2023)

ISO 11268-2:2023 土壤质量 污染物对蚯蚓的影响 第2部分:对蚯蚓繁殖的影响的测定

This document specifies one of the methods for evaluating the habitat function of soils and determining the effects of soil contaminants and chemicals on the reproduction of Eisenia fetida/Eisenia andrei by dermal and alimentary uptake. This chronic test is applicable to soils and soil materials of unknown quality, e.g. from contaminated sites, amended soils, soils after remediation, agricultural or other sites concerned, and waste materials. This method is designed mainly for determining the effects of soil contaminants and chemicals on the reproduction of Eisenia fetida/Eisenia andrei. Technical information is also provided on how to use Eisenia fetida/andrei for testing chemicals under tropical conditions (see Annex A). Finally, this method also includes technical information on how to use it with other environmentally relevant earthworm species: e.g. Dendrodrilus rubidus and Aporrectodea caliginosa (see Annexes B and C). This method does not apply to substances for which the air/soil partition coefficient is greater than one, or to substances with vapour pressure exceeding 300 Pa, at 25 °C. This method does not take into account the persistence of the substance during the test.

Soil quality — Effects of pollutants on earthworms — Part 2: Determination of effects on reproduction of Eisenia fetida/Eisenia andrei and other earthworm species

ASTM E1525-02(2023) 用沉积物设计生物试验的标准指南

1.1 As the contamination of freshwater and saltwater ecosystems continues to be reduced through the implementation of regulations governing both point and non-point source discharges, there is a growing emphasis and concern regarding historical inputs and their influence on water and sediment quality. Many locations in urban areas exhibit significant sediment contamination, which poses a continual and longterm threat to the functional condition of benthic communities and other species inhabiting these areas (1).2 Benthic communities are an important component of many ecosystems and alterations of these communities may affect water-column and nonaquatic species. 1.2 Biological tests with sediments are an efficient means for evaluating sediment contamination because they provide information complementary to chemical characterizations and ecological surveys (2). Acute sediment toxicity tests can be used as screening tools in the early phase of an assessment hierarchy that ultimately could include chemical measurements or bioaccumulation and chronic toxicity tests. Sediment tests have been applied in both saltwater and freshwater environments (2-6). Sediment tests have been used for dredge material permitting, site ranking for remediation, recovery studies following management actions, and trend monitoring. A particularly important application is for establishing contaminantspecific effects and the processes controlling contaminant bioavailability(7). 1.3 This guide is arranged as follows: Section Referenced Documents 2 Terminology 3 Application 4 Summary of Guide 5 Significance and Use 6 Hazards 7 Sediment Test Types 8 Biological Responses 9 Test Organisms 10 Experimental Design Considerations 11 Data Interpretation 12 Keywords 13 1.4 The values stated in SI units are to be regarded as the standard. The values given in parentheses are for information only. 1.5 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. For specific hazard statements, see Section 7. 1.6 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

Standard Guide for Designing Biological Tests with Sediments

BS ISO 23265:2022 土壤质量 污染土壤中有机物分解的估算试验

1   Scope This document specifies a test procedure for the evaluation of the habitat function of soils by determining effects of soil contaminants and substances on organic matter decomposition. This test is applicable to natural soils and soil materials of unknown quality (e.g. contaminated sites, amended soils, soils after remediation, agricultural or other sites under concern). This document also specifies how to use this method for testing substances under temperate conditions. This document is not applicable to substances for which the air/soil partition coefficient is greater than 1. It is not applicable to substances with vapour pressure exceeding 300 Pa at 25 °C. NOTE The stability of the test substance cannot be ensured over the test period. No provision is made in the test method for monitoring the persistence of the substance under test.

Soil quality. Test for estimating organic matter decomposition in contaminated soil

ISO 23265:2022 土壤质量.估算污染土壤中有机物分解的试验

This document specifies a test procedure for the evaluation of the habitat function of soils by determining effects of soil contaminants and substances on organic matter decomposition. This test is applicable to natural soils and soil materials of unknown quality (e. g. contaminated sites, amended soils, soils after remediation, agricultural or other sites under concern) . This document also specifies how to use this method for tes ting subs tances under temperate conditions. This document is not applicable to substances for which the air/soil partition coefficient is greater than 1 . It is not applicable to subs tances with vapour pressure exceeding 300 Pa at 25 °C . NO TE The s tability of the test subs tance cannot be ensured over the test period. No provision is made in the test method for monitoring the persis tence of the subs tance under tes t.

Soil quality — Test for estimating organic matter decomposition in contaminated soil

22/30439792 DC BS EN ISO 11267 土壤质量 土壤污染物对跳虫（Folsomia candida）繁殖的抑制

BS EN ISO 11267. Soil quality. Inhibition of reproduction of Collembola (Folsomia candida) by soil contaminants

ISO 23611-4:2022 土壤质量.土壤无脊椎动物的取样.第4部分:土壤寄生线虫的取样、提取和识别

Soil quality — Sampling of soil invertebrates — Part 4: Sampling, extraction and identification of soil-inhabiting nematodes

ASTM E2172-22 利用线虫 (Caenorhabditis elegans) 进行实验室土壤毒性试验的标准指南

1.1 This guide covers procedures for obtaining laboratory data to evaluate the adverse effects of chemicals associated with soil to nematodes from soil toxicity tests. This standard is based on a modification to Guide E1676. The methods are designed to assess lethal or sublethal toxic effects on nematodes in short-term tests in terrestrial systems. Soils to be tested may be (1) references soils or potentially toxic soil sites; (2) artificial, reference, or site soils spiked with compounds; (3) site soils diluted with reference soils; or (4) site or reference soils diluted with artificial soil. Test procedures are described for the species Caenorhabditis elegans (see Annex A1). Methods described in this guide may also be useful for conducting soil toxicity tests with other terrestrial species, although modifications may be necessary. 1.2 Summary of Previous Studies—Initial soil toxicity testing using the free-living, bacterivorous soil nematode Caenorhabditis elegans was developed by Donkin and Dusenbery (1).2 Following the development of an effective method of recovery of C. elegans from test soils, the organism was used to identify factors that affect the toxicity of zinc, cadmium, copper, and lead (2). Freeman et al. further refined the nematode bioassay by decreasing the quantity of soil and spiking solution volumes, determining test acceptability criteria, and developing control charts to assess worm health using copper as a reference toxicant (3). More recently, the toxicological effects of nitrate and chloride metallic salts in two natural soils were compared (4). LC50 values for C. elegans exposed for 24-h to nitrate salts of cadmium, copper, zinc, lead and nickel in an artificial soil (see Annex A2) were found to be similar to LC50 values for the earthworm, Eisenia fetida (5). Increasing the exposure time to 48-h resulted in much lower LC50 values (6). However, longer exposure times necessitate the addition of food and lead to lower recovery percentages in soils high in organic matter. A modification of the recovery method has also been used with a transgenic strain of C. elegans used as a soil biomonitoring tool to assess sub-lethal effects of metal exposures in soil (7). A variety of sub-lethal endpoints have been developed using C. elegans in aquatic media and may prove useful for assessing soil exposures (8). 1.3 Modification of these procedures might be justified by special needs. The results of tests conducted using typical procedures may not be comparable to results using this guide. Comparison of results obtained using modified and unmodified versions of these procedures might provide useful information concerning new concepts and procedures for conducting soil toxicity tests with terrestrial worms. 1.4 The results from field-collected soils used in toxicity tests to determine a spatial or temporal distribution of soil toxicity may be reported in terms of the biological effects on survival or sublethal endpoints. These procedures can be used with appropriate modifications to conduct soil toxicity tests when factors such as temperature, pH, and soil characteristics (for example, particle size, organic matter content, and clay content) are of interest or when there is a need to test such materials as sewage sludge. These methods might also be useful for conducting bioaccumulation tests. 1.5 The results of toxicity tests with (1) materials (for example, chemicals or waste mixtures) added experimentally to artificial soil, reference soils, or site soils, (2) site soils diluted with reference soils, and (3) site or reference soils diluted with artificial soil, so as to create a series of concentrations, may be reported in terms of an LC50 (median lethal concentration) and sometimes an EC50 (median effect concentration). 1.6 This guide is arranged as follows: Scope 1 Referenced Documents 2 Terminology 3 Summary of Guide 4 Significance and Use 5 Interferences 6 Apparatus 7 Safety Precautions 8 Soil 9 Test Organism 10 Procedure 11 1 This guide is under the jurisdiction of ASTM Committee E50 on Environmental Assessment, Risk Management and Corrective Action and is the direct responsibility of Subcommittee E50.47 on Biological Effects and Environmental Fate. Current edition approved Aug. 1, 2022. Published September 2022. Originally approved in 2002. Last previous edition approved in 2014 as E2172–01(2014). DOI: 10.1520/E2172-22. 2 The boldface numbers in parentheses refer to the list of references at the end of this standard. Copyright © ASTM International, 100 Barr Harbor Drive, PO Box C700, West Conshohocken, PA 19428-2959. United States This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee. 1 Analytical Methodology 12 Acceptability of Test 13 Calculation of Results 14 Report 15 Annexes A1. Caenorhabditis elegans A2. Artificial Soil Composition References 1.7 The values stated in SI units are to be regarded as the standard. 1.8 This standard does not purport to address all of the safety concerns, if any, associated with its use. It is the responsibility of the user of this standard to establish appropriate safety, health, and environmental practices and determine the applicability of regulatory limitations prior to use. While some safety considerations are included in this guide, it is beyond the scope of this standard to encompass all safety requirements necessary to conduct soil toxicity tests. Specific precautionary statements are given in Section 8. 1.9 This international standard was developed in accordance with internationally recognized principles on standardization established in the Decision on Principles for the Development of International Standards, Guides and Recommendations issued by the World Trade Organization Technical Barriers to Trade (TBT) Committee.

Standard Guide for Conducting Laboratory Soil Toxicity Tests with the Nematode Caenorhabditis elegans

KS I ISO 15952:2022 土壤质量.污染物对幼年陆地蜗牛（蜗牛科）的影响.土壤污染对生长影响的测定

Soil quality — Effects of pollutants on juvenile land snails(Helicidae) —Determination of the effects on growth by soil contamination