Journal of Chromatography A, 1259 (2012) 3–15
Recent developments in liquid chromatography–mass spectrometry and related techniques
Michal Holˇcapek, Robert Jirásko, Miroslav Lísa
Department of Analytical Chemistry, Faculty of Chemical Technology, University of Pardubice, Studentská 573, 53210 Pardubice, Czech Republic
Abstract
综述了在过去10年LC-MS和相关的技术进展。初学者或者中等经验的LC-MS使用者常会被市场上数量繁多的、不同的LC-MS系统所困扰,因此这篇文章综述了主要生产厂家的LC-MS及MALDI-MS的配置、设计,并从第三方评估其优点和缺陷。包括当前质量分析器、离子化技术、快速LC-MS、LC-MALDI-MS、用于LC-MS中的离子迁移技术,质谱的定量特点等趋势,也在这里讨论了一些新兴的质谱方法。
This review summarizes the state-of-art in liquid chromatography–mass spectrometry (LC–MS) and related techniques with the main focus on recent developments in the last decade. LC–MS records an enormous growth in recent years due to the application potential in analytical chemistry, biochemistry, pharmaceutical analysis, clinical analysis and many other fields, where the qualitative and quantitative characterization of complex organic, bioorganic and organometallic mixtures is needed. Beginners and moderately experienced LC–MS users may be confused by the number of different LC–MS systems on the
market, therefore an actual overview of mass spectrometers designed for the LC–MS configuration and matrix-assisted laser desorption/ionization mass spectrometry (MALDI-MS) from main manufacturers is compiled here together with an independent assessment of their advantages and limitations. Current trends in terms of mass analyzers, ionization techniques, fast LC–MS, LC–MALDI-MS, ion mobility spec-
trometry used in LC–MS, quantitation issues specific to MS and emerging mass spectrometric approaches complementary to LC–MS are discussed as well.