从入门到精通，SFC于医药工业之应用

超临界流体色谱技术 (Supercritical fluid chromatography, SFC) ，顾名思义，即流动相既不是气体也不是液体，而是使用超过临界温度和临界压力下的单一态流体(Supercritical Fluid)作流动相进行分离分析、制备纯化的色谱技术。

SFC的原理与液相色谱的原理类似，然而利用超临界流体（通常为二氧化碳CO₂）取代有机溶剂或水作为主要流动相，从本质上来说是一种高效的正相色谱技术。作为流动相所使用的超临界流体CO₂具有独特的低粘度和高扩散性能而成为一种智能溶剂，因具有与液体相近的密度，对各种物质有较强的溶解性，难挥发性和热稳定性差的物质分析均适用；同时，超临界流体的粘度近于气体，比液体约低1～２个数量级，而它的扩散系数在气体和液体之间，因而具有较快的传质速度和较小的传质阻力，分离过程中峰形变窄、柱效增加、分析速度加快。由此可见，SFC扬HPLC与GC之长、避GC及HPLC之短，具有独特的优越性，应用前景极为广阔。

由于SFC采用天然“绿色”的超临界流体CO₂作为溶剂，无需有机溶剂回收处理，省事经济环保，节能降耗又可持续使用，因而SFC对生物医药、精细化工、生态环境、农业食品等行业具有独特的吸引力。

SFC在制药行业，尤其是手性药物开发领域，由于其能够有效地利用液相色谱中常用的手性固定相 (chiral stationary phase，CSP) 且与GC、HPLC体系中使用的检测器完全兼容， SFC是一种理想的手性分离方法。因为它运行速度更快，溶剂用量更少，样品后处理更简单，总体上来讲，SFC是一种更经济、更绿色的方法，在手性化合物分离方面性能卓越，大有超越高效液相色谱法（HPLC）之趋势，为分离纯化和手性制备分离的有益替代方案。

Ps：从入门到精通，SFC于医药工业之应用学习资料

2007年

J. David Pinkston, The Procter & Gamble Company   宝洁公司

SFC for Fun and Profit: 45 Years of Promise, Progress, and Phrustration

http://www.greenchemistrygroup.org/pdf/sciprog2007/2_david_pinkston.pdf

Geoffrey B Cox, Chiral Technologies, Inc

Solvent and stationary phase selectivity in enantioselective SFC

http://www.greenchemistrygroup.org/pdf/sciprog2007/11_geoffrey_cox.pdf

Dauh-Rurng Wu, Bristol-Myers Squibb Company   百时美施贵宝

Chiral Supercritical Fluid Chromatography in Drug Discovery: from Analytical to Multigram-Scale

http://www.greenchemistrygroup.org/pdf/sciprog2007/3_dauh-rurng_wu.pdf

Larry Miller, Matt Potter, Wes Barnhart, Kyung Gahm and John Eschelbach, Amgen   安进

Application of SFC in Pharmaceutical Discovery and Early Development

www.greenchemistrygroup.org/pdf/sciprog2007/08_larry_miller.pdf

Ling S. Xiao, Xinxin Han, James B. Murphy and Mary Gasper, Pfizer Global Research & Development   辉瑞

A Comparative Study of Fast Orthogonal Chiral Screening Methods by SFC and Normal Phase HPLC

http://www.greenchemistrygroup.org/pdf/sciprog2007/james_murphy_pfizer.pdf

2008年

Insights into chiral recognition mechanisms on varied stationary phases in supercritical fluid chromatography

http://www.univ-orleans.fr/icoa/communications/com2008/west.pdf

Larry Miller, Matt Potter and Ian Sebastian, Amgen, USA   安进

Evaluation of Operating Conditions in Analytical and Preparative SFC

http://www.greenchemistrygroup.org/pdf/sciprog2008/MI1745Miller.pdf

Fiona J Bell, AstraZeneca   阿斯利康

SFC At AstraZeneca Alderley Park

http://www.greenchemistrygroup.org/pdf/sciprog2008/Bell_Second_International_Conference_on_Packed-Column_SFC,_Switzerland,_Oct_1st-Oct_2nd_2008A0[1].pdf

Preparative Chiral Chromatography in Pharmaceutical R&D   安进

www.chiraltech.com/enews/MilleCT07pres.pdf

2009年

Christine Aurigemma, Pfizer   辉瑞

Breaking the Flow Rate Barrier: Utilization of High Flow Mass-Directed SFC for Pharmaceutical Applications

http://www.greenchemistrygroup.org/pdf/sciprog2009/SFC2009-Christine%20Aurigemma-Pfizer.pdf

Georg Diehl, Novartis   诺华

SFC-A Versatile Tool in the Analysis and Purification of Pharmaceutical Compounds

http://www.greenchemistrygroup.org/pdf/sciprog2009/SFC2009-Georg%20Diehl-Novartis.pdf

Mark Hayward, Lundbeck   丹麦灵北

SFC with Tandem Mass Spectrometry to Evaluate the in vivo Disposition of Individual Stereoisomers of Drugs

http://www.greenchemistrygroup.org/pdf/sciprog2009/SFC2009-Hayward-Lundbeck.pdf

Chiral Separations using SFC: Applications from Discovery through Development

http://sfc-ms.com/pdfs/webinars/chiral_sep_applic.pdf

A Screening Process for Development of Enantioselective Supercritical Fluid Chromatography Separation Methods

http://www.chiraltech.com/app/LCGC_MethDev.pdf

Dauh-Rurng Wu, Bristol Myers Squibb   百时美施贵宝

Preparative Chiral SFC in Drug Discovery: Promises and challenges

http://www.greenchemistrygroup.org/pdf/sciprog2009/SFC2009-Dauh-Rurng%20Wu-BMS.pdf

Larry Miller, Amgen   安进

Evaluation of Non-Traditional Co-Solvents for Routine Pharma Discovery Support

http://www.greenchemistrygroup.org/pdf/sciprog2009/SFC2009-Larry%20Miller-Amgen.pdf

Zhenyu Wang, Schering-Plough   先灵葆雅

SFC-a Powerful Tool for Polymethoxyflavone Isolation and Analysis

http://www.greenchemistrygroup.org/pdf/sciprog2009/SFC2009-Zhenyu%20Wang-Schering-Plough.pdf

声明：上述SFC相关资料，来自 2007、2008、2009 SFC International Conference，仅供学习参考，勿作它用，谢谢！