使用在线TurboFlow技术定量人体血液和尿液中苯二氮卓类药物

前言：

Develop a simple, fast, robust and cost-effective LC/MS method for automated extraction and confirmatory quantitation of benzodiazepines in human blood and urine for clinical and forensic applications.

仪器：

Thermo Scientific Transcend TLX system

结论：

A simple, rapid and robust quantitative LC/MS assay for benzodiazepines in biological fluids was developed. Online extraction, separation, detection and quantitation of 23 benzodiazepines in human plasma and urine were achieved in 20 minutes using the Transcend TLX system coupled to the MSQ Plus Single Quadrupole Mass Detector. The linear dynamic range obtained was 5–1000 ng/mL in matrix. Consistent ppb level quantitative results with primary ion and confirmative ion in blood and urine matrices with peak area RSD better than 5% were obtained. The quantitative results with confirmative ion on three post-mortem blood samples and two post-mortem urine samples were in excellent agreement with primary ion quantitative results.

TurboFlow technology drastically reduced sample preparation, eliminated matrix effects and significantly improved sensitivity to allow robust confirmatory quantitation of low ppb levels of benzodiazepines with single quadrupole mass spectrometry. Convenient and cost-effective, the automated benchtop TLX-MSQ LC/MS platform is ideally suited for routine use in clinical and forensic toxicology applications.