SCIEX 宣布与 IROA 达成联合营销协议, 以改进非靶向代谢组学复杂样品中的代谢物识别和定量

SCIEX 和 IROA 技术有限责任公司为下一代代谢组学提供完整的解决方案, 允许在样本中对代谢物进行无歧义的识别和定量

2018年2月5日

全球领先的生命科学分析技术公司--SCIEX 今天宣布与 IROA Technologies 公司 (一家专门从事代谢组学分析的试剂和试剂盒公司) 达成合作营销协议。这种关系将使 SCIEX 处于独特的地位, 通过使生物系统中的生化物质测量能够并自动实现其自动化, 来应对非目标代谢组学分析方面的一个重大挑战, 即代谢物的识别。

根据本协议, IROA 技术和 SCIEX 将推出一个独特的标签套件, 称为 ILO 工作流套件, 基于下一代代谢组学和®系统的 SATH®采集。这种对改进代谢物识别的关注, 同时提高定量准确性, 将使科学家能够区分真正的生化特征和噪音, 从而提高他们对复杂样本类型分析的信心。产生卓越的生物学见解和发现。

IROA®是一种同位素方法, 其中所有生物分子都被统一和随机标记, 以创建易于从伪影中区分的信息丰富的同位素模式。由于标签的一致性, 这些图案不仅出现在 MS 中, 而且还出现在任何后续的 msp 中的所有片段中。

同位素比异常分析 IROA 是一种简单而直接的代谢分析技术, 在这种技术中, 特定和独特的特征或 (标记) 被嵌入到生化代谢物中, 通过质谱 (MS) 进行相对定量, 并使用软件用于查找、识别和量化这些特定签名的算法。

"SWAH 和 IROA 的结合通过支持13C 同位素标准, 增强了已知代谢物和未知代谢物的识别, 从而改善了全球代谢组工作流程, 并最终将提高数百种代谢物的定量性能。代谢物在一次注射, "蒂姆加勒特博士, 副教授, 在大学佛罗里达医学院。

SATH 采集是一种独立于数据的采集 (DIA) 工作流, 在定量发现蛋白质组学中得到了很好的应用, 现在开始在非目标代谢组学工作流中取得进展。SATH 允许用户收集每个可检测到的峰值的 MS 和 MSMS, 从而创建代谢组的数字地图。可变窗口 SWATH (一种收集 MSMS 的增强方法, 在 MS 频谱的密集区域中使用较窄的质量窗口) 允许特异性。主要优点是, 通过消除背景噪声和只保留与生物相关的峰值, 从而消除错误发现, 从而减少数据质量, 从而大大提高了数据质量。

SCIEX 战略市场管理总监马克·卡法佐说: "通过在大型样本中对数百种代谢物进行可重复量化来推进精确医学, 对学术和临床研究实验室都非常重要。"使用具有可变窗口 sath 采集的 IROA, 研究人员可以从复杂混合物中收集全面的数据, 以便在一次注射中进行识别和定量, 然后快速降低结果的复杂性, 从而加速与生物有关的代谢物的鉴定。通过与 IROA 技术公司合作, 并将 IROA 工作流工具包添加到 SCIEX 下一代代谢组学解决方案中, 我们帮助科学家解决他们每天面临的代谢物识别挑战, 尤其是在处理复杂样本时。

SCIEX Announces Co-Marketing Agreement with IROA Technologies LLC to Improve Metabolite Identification & Quantitation in Complex Samples for Untargeted Metabolomics

SCIEX and IROA Technologies LLC to Offer Complete Solution for Next Generation Metabolomics, to Allow Unambiguous Identification and Quantitation of Metabolites in a sample

February 05, 2018

Framingham, MA — SCIEX, a global leader in life science analytical technologies, today announced a co-marketing agreement with IROA Technologies LLC, a reagents and kits company specializing in metabolomics analyses. This relationship will uniquely position SCIEX to address a major challenge in untargeted metabolomics analyses, namely metabolite identification, by enabling and automating the measurement of biochemicals in biological systems.

Under this Agreement, IROA Technologies and SCIEX will promote a unique labeling kit called the IROA Workflow Kit with SWATH® Acquisition based on next-generation metabolomics and TripleTOF® Systems. This focus on improving metabolite identification while improving quantitative accuracy will enable scientists to differentiate the real biochemical signatures from the noise allowing higher confidence in their analyses of complex sample types, potentially yielding superior biological insights and discoveries.

IROA® is an isotopic methodology in which all biological molecules are uniformly and randomly labelled to create informative isotopic patterns that are readily discriminated from artifacts. Because of the uniform nature of the labelling, these patterns show up in not only the MS, but also in all fragments in any subsequent MS/MS.

Isotopic Ratio Outlier Analysis IROA is a simple and direct metabolic profiling technique in which specific and unique signatures or (tags) are embedded into biochemical metabolites for relative quantitation by mass spectrometry (MS) and uses software algorithms to find, identify and quantitate these specific signatures.

"The combination of SWATH and IROA improves global metabolomic workflows by enabling reference to a 13C isotopic standard, enhancing the identification of both known and unknown metabolites, and will ultimately improve quantitative performance of hundreds of metabolites in a single injection," states Dr. Tim Garrett, Associate Professor at University Florida School of Medicine.

SWATH Acquisition, a data independent acquisition (DIA) workflow is well adopted in quantitative discovery proteomics, and is now starting to take off in untargeted metabolomics workflows. SWATH allows a user to collect MS and MSMS of every detectable peak in their sample, thus creating a digital map of the metabolome. Variable Window SWATH (an enhanced way of collecting MSMS, using narrower mass windows in denser regions of the MS spectrum) allows for specificity. The major advantage is that there is a huge enhancement of data quality through data reduction by removal of background/noise and keeping only the biologically relevant peaks, thereby eliminating false discovery.

"Advancing precision medicine by reproducibly quantifying hundreds of metabolites in large sample cohorts is of high importance to both academic and clinical research labs," said Mark Cafazzo, Director of Strategic Market Management at SCIEX. "The use of IROA with variable window SWATH Acquisition allows researchers to collect comprehensive data from complex mixtures for identification and quantitation in a single injection, and then rapidly reduce the complexity of the results, accelerating the identification of biologically-relevant metabolites. By partnering with IROA Technologies and adding the IROA Workflow Kit to SCIEX next-generation metabolomics solutions, we help scientists address the metabolite identification challenges they face every day, especially when working with complex samples."