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MCL单分子成像 微镜TIRF系统

参考报价: 面议 型号: MicroMirror TIRF System
品牌: MCL 产地: 美国
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Features
  • Integrated imaging & motion control platform

  • Spatially separated excitation & emission beams

  • Adjustable micromirror mounts

  • Open access to optical pathways

  • Highly stable, yet flexible, platform

  • Integrated slide holder

  • TIRF Lock feedback control

Advantages
  • Superior signal-to-noise ratio

  • Eliminates dichroic mirrors

  • Optical pathway accessibility

  • Facilitates multiple laser lines

  • Proven design

  • Enabling technology for studying complex biomolecular interactions




Product Description

The technique of micro-mirror TIRF (total internal reflection fluorescence) microscopy is the only proven method to study the ordered assembly and function of multi-component biomolecular machines. The Mad City Labs' MicroMirror TIRF system is part of our single molecule imaging instrument portfolio and draws on our expertise with high precision, high stability nanopositioning systems and microscopy solutions.

The MicroMirror TIRF system uses through-the-objective excitation, but replaces the dichroic used in conventional TIRF systems with two broadband micro-mirrors positioned at the back aperture of the objective lens. The elimination of the dichroic mirror leads to superior signal-to-noise ratios and simplifies the introduction of multiple spectral lines to your experiment. Typical biomolecular systems studied using micro-mirror TIRF incorporate 3 or more laser lines which can be difficult to accomodate in conventional dichroic TIRF instruments.

Each micro-mirror is mounted to a multi-axis precision mount allowing the user to make fine adjustments to the optical pathway. The imaging and motion control platform is designed to maximize optical pathway accessibility and flexibility, while also simplifying the optical alignment. A compelling advantage of our MicroMirror TIRF system is the open access to the entry and exit optical pathways. The motion control platform comprises an XY micropositioning platform with an integrated XYZ closed loop nanopositioning system. The XYZ closed loop nanopositioning uses proprietary PicoQ® sensors for sub-nanometer precision and high stability. A z-axis micropositioner with integrated sample holder is mounted above the nanopositioning system.

Completing our MicroMirror TIRF system is the TIRF Lock™ feedback control consisting of a QPD sensor, TIRF Lock™ controller and software. TIRF Lock™ maintains the TIR signal through software feedback to the nanopositioner.

The MicroMirror TIRF system is an enabling technology for advanced study of complex biomolecular interactions. The MicroMirror TIRF is a proven design and offers a simple yet flexible instrument platform for single molecule research.

Compatible Software Packages:
      





Technical Specifications - Imaging & Motion Control

Micropositioning motionXYZ
Ranges of motion (XY)
25 mm
Range of motion (Z)10 mm
Body MaterialAnodized Aluminum
Nanpositioning MotionXYZ
Ranges of motion (XYZ)200 μm
Resolution0.4 nm
Recommended max. load (horizontal)*0.5 kg
Body MaterialAluminum
Controller?Nano-Drive®
Digital InterfaceUSB 2.0
Analog Input0 to 10V
Synchronization outputsTTL (4)
Nanopositioner Software?Nano-Route®3D
TIRF Software compatibilityGLIMPSE


Technical Specifications - MicroMirror Mounts

Micro-Mirror motion5 axis
Micro-Mirror diameter3 mm
Mount materialAluminum



Technical Specifications - TIRF Lock™ Feedback

TIRF-Lock™ SensorQuadrant Photodiode
ControllerTIRF Lock
Digital interfaceUSB 2.0
SoftwareTIRF Lock


* Larger load requirements should be discussed with our engineering staff.
? See Software Compatibility, above



Additional Information

MicroMirror TIRF Catalog Pages
Design and construction of a multiwavelength, micromirror total internal reflectance fluorescence microscope. Larson, J. et al. Nature Protocols 9, 2317–2328 (2014)



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注:该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案,不可用于临床诊断或治疗等相关用途

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