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poster
Mass Spectrometry-Based Imaging Approaches to Spatial Localisation of Drugs in Tissue
Aim
To compare two approaches to imaging mass spectrometry for localisation of a drug,AZ-1, in rodent tissue samples. Important experimental considerations such as spatial resolution, time of acquisition and fit-forpurpose assessment were evaluated.
Methods
Matrix-assisted laser desorption/ionisation (MALDI) and liquid extraction surface analysis(LESA), coupled to a Waters Synapt G2 HDMS travelling wave ion mobility mass spectrometer.
Results
AZ-1 was successfully identified in dosed tissue slices by means of LESA-MS/MS and MALDIMS/MS experiments. Extra selectivity of an MS/MS experiment was required to identify the drug compound, due to the complexion mixture present in tissue sections.
Conclusions
Whilst both techniques can be used to analyse tissue sections, method choice will ultimately depend on the compound of interest within the tissue and spatial resolution required for the experiment.