在大鼠肝细胞–使用LC MS和LC–MS2进行的(±)-4_-methylmethcathinone (4-MMC)和 (±)-4_-（三氟甲基）甲卡西酮（4-TFMMC）的第一阶段和第二阶段的代谢途径的研究

　　摘要：(±)-4_-Methylmethcathinone hydrochloride [(±)-mephedrone, 4-MMC] is a synthetic ‘legal high’, with a classical cathinone structure similar to methcathinone. In this study, the in vitro metabolism of 4-MMC was investigated in Sprague-Dawley rat hepatocytes to characterise the associated Phase I and II metabolites. 4-MMC was incubated with rat liver hepatocytes, and the reaction mixture was analysed on a zwitterionic hydrophilic interaction (ZIC-HILIC) column using LC–MS and LC–MS2. 4-MMC was metabolised, yielding 17 metabolites. These metabolites were structurally characterised on the basis of accurate mass analyses and LC–MS2 fragmentation patterns and the major metabolic routes for 4-MMC determined to be via (i) oxidation of the 4’-methyl group and (ii) reduction of the β-keto moiety. The biotransformation of a modified 4’-trifluoromethyl- derivative (4-TFMMC) has also been studied and shows significant differences in its metabolism compared to 4-MMC. Key pharmacokinetic parameters for both drugs have been calculated [biological half-lives (t1/2) for 4-MMC = 61.9 min and for 4-TFMMC = 203.8 min] and this data may aid in the understanding of in vivo metabolism and the likely pharmacokinetic effects of chemical/structural modifications within this class of controlled substances.

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