三重四极杆液质联用系统测定谷类和谷类食品中的17种真菌毒素

      Mycotoxins are potentially toxic secondary metabolites produced by different species of fungi. The main relevant species occurring on cereals are Penicillium sp., Fusarium sp. and Aspergillus sp. The fungal contamination can either occur on the field or during storage. Estimations are given that approximately 25% of all crops are contaminated with mycotoxins. The type and amount of contamination varies with climate conditions. As the contamination can result in serious health issues,mycotoxin analysis is an important part of routine food control.1, 2

      For cereals and baby food, the European Union (EU) legislation currently sets maximum levels (ML) for the following mycotoxins: deoxynivalenol, zearalenone, T-2 

toxin, HT-2 toxin, fumonisin B1, fumonisin B2, aflatoxin B1, B2, G1, G2 and ochratoxin A.3-6 The European Food Safety Authority (EFSA) is continuously examining the occurrence and health risks of further mycotoxins. One of the main current topics of interest is the acetylated deoxynivalenol metabolites and deoxynivalenol-3 glucoside.7 For some mycotoxins, e.g. sterigmatocystin, which is related to aflatoxins, EFSA could not determine the risk due to lack of data.8

      In our measurements, matrix effects were estimated by comparing the slope of the calibration curve in solvent and matrix (see Table 4). Even after dilution of extract, 

most analytes showed medium to high matrix effects. The matrix effects in maize are generally higher than in wheat. Thus, for this approach a matrix matched calibration for quantitation is still highly recommended. The linearity was reviewed for solvent as well as for the two matrices. The values are summarized in Table 4 and show good 

linearity (>0.99) for most analytes in solvent and matrix. The injection precision was determined by six replicate 

      injections of a standard in solvent, wheat and maize. The relative standard deviation (RSD) in % is summarized in Table 5.