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Simultaneous Metabolite Identification and Quantitation of a Parent Drug Using Reverse Energy Ramp Scanning on a Triple Stage Quadrupole Mass Spectrometer |
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Quantitation-Enhanced Data-Dependent MS/MS scanning on a triple stage quadrupole mass spectrometer enables simultaneous metabolite identification and quantitation of a parent drug. The selectivity provided by this technique helps to increase the detection of metabolites. When the QED-MS/MS and RER techniques are coupled, the structure and concentration profiles of parent drugs and metabolites can be obtained simultaneously, thus increasing the bioanalytical throughput in drug discovery laboratories.