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样品: | Bovine brain, heart, liver,yeast | 项目: | Lipid |
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Higher Resolution LCMS and MSMS Analysis of Lipid Extracts Using Benchtop Orbitrap-based Mass Spectrometers and LipidSearch Software |
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Four different total lipid extracts were profiled and are suitable for benchmarking lipidomics experiments, separation conditions and instrumental optimization. Higher resolution and speed of Q Exactive HF provides more confident identification and relative quantification in a single positive and negative ion run. At least 20% more lipid ID’s were obtained using a ultra-high field benchtop Orbitrap. LipidSearch provides automated identification of more than 500 lipid species in a single LC-ddMS2 experiment, and alignment of multiple samples gives over 1000 lipid species.