Stem Cell Transcription Factors ChIP PCR Array The Human Stem Cell Transcription Factors EpiTect Chip qPCR Array profiles modified histone and nuclear protein binding to the proximal promoter of a panel of transcription factor genes associated with stem cell differentiation and development. The array contains 96 pairs of qPCR primers targeting the 1-kb region downstream of the transcription start sites (TSS) of 84 biological important genes plus 12 appropriate control regions. The classes of transcription factors represented by this array include forkhead box, GATA binding, homeobox, paired box, POU class, SOX, hormone receptors, oncogenes, tumor suppressor genes and the transcription factors required for induced pluripotency. Profiling the histone modifications and nuclear protein binding events at these gene promoters in your stem cells or differentiated cells will help you correlate these interactions with pluripotency and differentiation status. The results can also provide insights into the epigenetic molecular mechanisms and biological pathways behind development and differentiation, such as signal transduction. Using Chromatin Immunoprecipitation and this real-time PCR Array, you can easily and reliably analyze the association between your chosen nuclear factors and the promoters for a focused gene panel involved in stem cell differentiation and development.    

Chromatin Modification and Remodeling: DNMT3B, EZH2, TERT.
Forkhead Box Proteins: FOXA1, FOXA2, FOXP1, FOXP2, FOXP3.
GATA Binding Proteins: GATA1, GATA6.
Homeobox Genes: CDX2, DLX1, DLX2, HOXA10, HOXA11, HOXA2, HOXA3, HOXA7, HOXA9, HOXB1, HOXB13, HOXB3, HOXB5, HOXB8, HOXC10, HOXC12, HOXC4, HOXC5, HOXC6, HOXC9, HOXD1, HOXD10, HOXD4, IRX4, ISL1, LMX1B, MSX2, NANOG, NKX2-2, PITX2, PITX3, SIX2, TLX3.
Induced Pluripotency Transcription Factors: NANOG, MYC, POU5F1, SOX2.
Kruppel-Like Factors: KLF2, KLF4.
Nuclear Hormone Receptors: ESR1, HTR7, NR2F2, PPARG, VDR.
Oncogenes & Tumor Suppressor Genes: JUN, MYC, RB1, WT1.
Paired Box Genes: PAX1, PAX5, PAX6, PAX9, PITX2, PITX3.
POU Class Homeobox Genes: POU4F1, POU4F2, POU5F1.
Signal Transduction: GLI2, NFATC1, NOTCH2, SMAD2, STAT1, STAT3.
SRY (Sex Determining Region Y)-Box Genes: SOX2, SOX6, SOX9.
Other Transcription Factors: DACH1, EGR3, HAND1, NEUROD1, OLIG2, RUNX1, SP1, TBX5, ZFPM2, ZIC1.
Other Genes: LIN28B, PCNA, TDGF1, WRN.

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