清华大学与清谱科技合作研发小型质谱技术，发表Analytical Chemistry封面文章

     清华大学精密仪器系欧阳证教授团队与清谱科技长期合作研发小型质谱技术，近期成果于2019年1月15日以封面文章发表在Analytical Chemistry，题为“Tandem Analysis by a Dual-Trap Miniature Mass Spectrometer”。此篇论文中，作者研制了采用双线型离子阱（LIT）结构的小型质谱仪，实现传统大型三重四级杆质谱仪的分析功能。

     质谱小型化将极大促进未来质谱分析走进人们日常生活，是当今质谱仪器研发的主要技术方向之一。考虑到现场用户往往没有足够的分析经验，小质谱的易用性也成为了扩展质谱应用范围的重要因素。一个典型的例子就是在POC分析应用中，医生需要使用规范的简单操作对病人进行检测，而不是多功能但复杂的实验室程序。所以无需样品处理的原位电离质谱应运而生，为直接分析复杂样品提供了一种有效途径。在临床检测及食药检测中往往需要判定分析物的精确含量，这就对小型质谱仪的定量能力提出了更高的要求。

      质谱小型化和原位电离也是国内学者积极参与并引领的学术领域之一。清华精密仪器系的质谱研发团队以发展非连续大气压接口小型离子阱质谱仪技术及原位采样离子化方法为技术方向，多年来研发多个关键技术及小型仪器，包括首个手提式质谱仪(Mini 10) ^[1]，首个常压源小型质谱（Mini 10）^[2]，最轻（4kg）自给式手持质谱仪^[3]，首台可用于POCT的质谱仪（Mini 12）^[4]，低温等离子源（LTP）^[5]及纸喷雾（Paper Spray）^[6]等。最近，该团队与上海华山医院及普渡大学合作，将小质谱应用于神经胶质瘤的诊断^[7]。

     此次工作，采用清谱科技的Mini 仪器平台，使用双线型离子阱及适时气压控制，实现离子阱间高效的选择性传输及多模式（in-trap和beam-type）碰撞诱导解离(CID)；使得通常只有在大型三重四极杆质谱仪才可完成的功能，如单反应监测（SRM），多反应监测(MRM)及母离子扫描等，可由台式计算机大小的质谱仪实现。对于未来POCT及其他现场分析应用中复杂样品的高精度定量，及生物标记物的高特异性鉴定，意义非凡。

[参考文章]：
[1]、Liang Gao, Qingyu Song, Garth E. Patterson, R. Graham Cooks, and Zheng Ouyang, "Handheld Rectilinear Ion Trap Mass Spectrometer" Analytical Chemistry 2006 78(17) , 5994 -6002, doi: 10.1021/ac061144k
链接地址：
https://pubs.acs.org/doi/pdf/10.1021/ac061144k
[2]、Liang Gao, R. Graham Cooks, Zheng Ouyang, "Breaking the Pumping Speed Barrier in Mass Spectrometry: Discontinuous Atmospheric Pressure Interface" , Analytical Chemistry , 2008 , 80, 4026-4032, doi:10.1021/ac800014v
链接地址：
https://pubs.acs.org/doi/10.1021/ac800014v
[3]、Liang Gao, Andy Sugiarto, Jason D. Harper, R. Graham Cooks, Zheng Ouyang, "Design and Characterization of a Multisource Hand-Held Tandem Mass Spectrometer", Analytical Chemistry, 2008, 80 (19), pp 7198–7205, Accelerated Article, doi: 10.1021/ac801275x
链接地址：
https://pubs.acs.org/doi/10.1021/ac801275x
[4]、L. Li, T.-C. Chen, Y. Ren, P. I. Hendricks, R. G. Cooks and Z. Ouyang; "Mini 12, Miniature Mass Spectrometer for Clinical and Other Applications—Introduction and Characterization", Analytical Chemistry, 2014, 86 (6), pp 2909–2916, DOI: 10.1021/ac403766c
链接地址：
https://pubs.acs.org/doi/10.1021/ac403766c 
[5]、Jason D. Harper, Nicholas A.Charipar, Christopher C. Mulligan, R. Graham Cooks, and Zheng Ouyang, "Low Temperature Plasma Probe for Ambient Desorption Ionization", Analytical Chemistry, 2008, 80 (23), pp 9097–9104, doi:10.1021/ac801641a
链接地址：
https://pubs.acs.org/doi/10.1021/ac801641a
[6]、He Wang; Jiangjiang Liu.; R. Graham Cooks; Zheng Ouyang; "Paper Spray for Direct Analysis of Complex Mixtures Using Mass Spectrometry", Angewandte Chemie International Edition, 2010, 49, 877-880, doi: 10.1002/anie.200906314
链接地址：
https://onlinelibrary.wiley.com/doi/10.1002/anie.200906314
[7]Zou R, Cao W, Chong L, et. al. “Point-of-Care Tissue Analysis Using Miniature Mass Spectrometer” [J]. Analytical Chemistry, 2019, 91(1): 1157-1163.
链接地址：
https://pubs.acs.org/doi/10.1021/acs.analchem.8b04935

可通过以下链接查看全文：https://pubs.acs.org/doi/ipdf/10.1021/acs.analchem.8b03958

【文章摘要】
Miniature mass spectrometers are of an increasing interest for in-situ analyses, and their coupling with the ambient ionization sources is a valid path for direct analysis of complex samples. In this study, a miniature mass spectrometer using discontinuous atmospheric pressure interface was developed with a dual-LIT (linear ion trap) configuration. The comprehensive scan modes were enabled for tandem mass spectrometry analysis, which are critical for high-quality qualitative and quantitative analysis. A real-time pressure control was implemented to facilitate the ion transfer and collision induced dissociation (CID). Beam-type CID could be performed for tandem analysis at a high number of stages. In-trap CID at high q could also be performed with the fragment ions accumulated in a second trap.A precursor ion scan mode for analyzing target analytes has also been demonstrated.