您好,欢迎您查看分析测试百科网,请问有什么帮助您的?

稍后再说 立即咨询
东方闪光(北京)光电科技有限公司
400-6699-117转1000
热门搜索:
分析测试百科网 > Semrock > 激光器 > Confotec CARS 系统

Confotec CARS 系统

参考报价: 面议 型号: Confotec CARS 系统
品牌: Semrock 产地: 德国
关注度: 9 信息完整度:
样本: 典型用户: 暂无
咨询留言 在线咨询

400-6699-1171000

AI问答
可以做哪些实验,检测什么? 可以用哪些耗材和试剂?

Confotec CARS 系统




多种同步分析功能:


  • 高速 CARS信号成像

  • 共聚焦拉曼成像

  • 共聚焦荧光成像,包含双光子荧光(或多光子)

  • 共聚焦激光反射成像

  • 高对比度激光透射成像







三种类型CARS测试:


  • F-CARS

  • E-CARS

  • P-CARS



五独立通道同步获取4种2D及3D图像


  • F-CARS

  • E-CARS及拉曼

  • 激光反射成像

  • 激光透射成像

  • 激光激发成像

  • 激发及探测偏振控制

  • 计算机控制全自动测试模式切换









三种扫描模式


  • XY阵镜扫描

  • XY自动位移台扫描

  • XY阵镜扫描+自动位移台扫描实现高分辨率全景成像

Confotec CARS 系统详细技术参数




Common specifications

Spatial XYZ resolution of CARS signals
(Objective lens: 60х, NA=1.2, watter immersion):
< 0.7 μm
Spatial resolution of Raman signals
(excitation wavelength 633 nm; objective lens: 60х, NA=1.2, watter immersion):
XY: < 300 nm
Z:   < 700 nm
Spectral detectable range:

CARS signals:            985 – 5000 cm-1
Raman signals:          75 – 6000 cm-1

Spectral resolution:CARS signals:                7-8 cm-1
Raman signals:              0.25 cm-1(grating 75 l/mm Echelle);
0.6 cm-1 (grating 1800 g/mm)
Scanning range (fast scanning mode, 60x lens)XY: 225 х 225 μm
Z:   80 μm
Control and automation:Fully motorized

Optical microscope 

Type:inverted
Model:Nikon Ti-U
Stage:motorized
- travel range:114 х 75 mm
- accuracy (for 1 mm traveling):0.06 μm
- XY repeatability:± 1 μm
- minimal step:0.02 μm
Micro- objective lens:60 х NA-1.2 water immersion
20 x NA-0.45
Z-scanner:
- type:piezo scanner
- lens movement range:80 μm
- minimal step:50 nm
- Reapetability:< 6 nm
Illuminator for reflection mode:Halogen lamp 100 W
Illuminator for transmission mode:LED
Laser input port:motorized triple turret
High resolution digital video camera:
- type:digital color CCD camera
- sensor:1/2", 2048 x 1536 pixeles
- ADC:10 bit,  12 frames per min


CARS excitation laser system

Single module optical parametric laser system consisting of:
Picosecond solit state Nd:YVO4 laser
Output wavelength:1064 nm
Pulse duration:6.5 ps
Output power:6 W
Pulse repetition rate:85 MHz
Mode composition:TEM00
Beam quality (М2):< 1.5
Beam diameter:2 mm
Tunable SOPO
(Synchronously pumped Optical Parametrical Oscillator)

Wavelength tuning range:690 - 990 nm
Output power:> 400 mW (at 800 nm)
Pulse duration:5 - 6 ps
Pulse repetition rate:85 MHz
Polarization:horizontal
Mode composition:TEM00
Beam quality (М2):< 1.2
Beam diameter:2 mm
Built-in delay tuning optical line

Laser for Raman spectroscopy

Laser type:cw HeNe
Wavelength:632.8 nm
Average output power:> 10 mW
Polarization:linear
Beam diameter:0.65 mm
Beam divergence:< 1.24 mrad


Laser beam convergence and appointment unit

Laser shutter:3 pieces (Nd:YVO4 laser, SOPO and HeNe laser)
Polarizer:2 pieces (Nd:YVO4 laser and SOPO)
Wavelength plate λ/22 pieces (Nd:YVO4 laser and HeNe laser)
Variable telescope:3 pices (Nd:YVO4 laser, SOPO and HeNe laser)


Main optical unit

Optimized optics for spectral range:400 - 1100 nm
Polarization analyzer:Glan-Teylor prism
Laser beam attenuator:veriable neutral density filter 0 - 3D
Pinhole lens positioner:three-axis (X, Y, Z)
CARS, Raman, fluorescence filters positioner:five- position
Posotioner of dihroic mirrors:six- position


Imaging monochromator/spectrograph MS5004i

Focal length:520 mm
F number (input):9.8
Horizontal magnification:1.0
Vertical magnification:1.0
Vertical spatial resolution:< 20 μm
Flat field size:28 х 5 mm
Stray light
( 20 nm from 633 nm laser line)
1 х 10-5
Difraction gratings unit:4-position motorized grating turret
Spectral resolution
(wavelength 633 nm, CCD pixel size: 12x12 μm)

0.01 nm (Echelle grating 75 l/mm)
0.025 nm (grating 1800 l/mm)

Entrance spectral slitmotorized confocal pinhole,
continuously adjustable from 0 to 1.5 mm
Output spectral slit:motorized, width from 0 to 2 mm (continuously adjustable)
Ports:1 input, 2 output
Detector (Raman, E-CARS, fast measurements)Hamamatsu Photosensor module H7844 (with TE cooling)


Monochromator for fluorescence measurements (integrated with MS5004i)

Focal length:100 mm
Input spectral slit:motorized confocal pinhole
Output spectral slit:fixed, width 4.2 mm
Difraction grating:600 l/mm
Spectral range:400 - 920 nm
Linear reciprocal dispersion:13 nm/mm
DetectorHamamatsu Photosensor module H7844 (with TE cooling)


Spectral CCD camera (Raman, E-CARS)

Type:digital CCD camera
Photosensor:back-thinned CCD sensor 2048 х 122 pixels
Pixel size:12 х 12 μm
Photosensitive area:24.576 х 1.464 mm (length х heigh)
Spectral sensitivity range:from 200 to 1100 nm
Cooling with temperature stabilization:two-stage Peltier (TE) cooling down to – 45 °С
ADC digitization:16 bit
Sensitivity:1 photon for 1 ADC count (at 650 nm)
Dinamic range:at least 10 000


Fast scanning unit (X, Y)

Scanners:Galvano-mirror scanners (X, Y)
Scanning model:raster high speed and start-stop mode
Positioning accuracy:< 30 μm
Scanning area:225 μm х 225 μm (objective lens 60Х)
Full frame scanning speed:4 s/frame: 1000 х 1000 points


Laser confocal microscope unit (Reflected)

Prepinhole lens positioner:three -axis (X, Y, Z)
Confocal pinhole:motorized confocal pinhole,
continuously adjustable from 0 to 1.5 mm
Detector:Hamamatsu Photosensor module H6780-01

F-CARS and transmitted signal registration system

Polarization alanyzer:Glan-Teylor prism
F-CARS filters positioner:4-position, motorized
F-CARS signal registration detector:Hamamatsu Photosensor module H7844 (with TE cooling)
Transmitted signal registration detector:Hamamatsu Photosensor module H6780-01


Registration and processing control unit

Number of registration channels:5
Number of simultaneously registered channels:up to 5
PC connection interface:Ethernet 100 Base-T (TCP/IP protocol)

Polysterene balls of various diameter (F-CARS, 3045 cm-1)



3D CARS image of liquid crystal 8CB structure on resonant frequency 2236 cm-1


Multimodal CARS/TPEF image of growing cancer cells HeLa: DNA/RNA/Proteins/Lipids colored


Selective imaging of MIA-PaCa pancreatic human cancer cell for resonance 2845 cm -1 C-H bound (C-H aliphatic stretch). Selective lipids visualization.


3D image of a cell

References:
Dr. A. V. Kachynski,
The Institute for Lasers, Photonics,
and Bio-photonics,
State University of New York at Buffalo



Confotec CARS 系统信息由东方闪光(北京)光电科技有限公司为您提供,如您想了解更多关于Confotec CARS 系统报价、型号、参数等信息,欢迎来电或留言咨询。

注:该产品未在中华人民共和国食品药品监督管理部门申请医疗器械注册和备案,不可用于临床诊断或治疗等相关用途

移动版: 资讯 直播 仪器谱

Copyright ©2007-2024 ANTPEDIA, All Rights Reserved

京ICP备07018254号 京公网安备1101085018 电信与信息服务业务经营许可证:京ICP证110310号