探索蛋白质组学前沿：Neil Kelleher教授分享最新进展｜WeOmics G28

“Westlake Proteomics Series” (WeOmics)系列研讨会 由The Proteomic Navigator of the Human Body (π-HuB) Project，CN-HUPO，西湖大学Guomics实验室，西湖实验室iMarker实验室共同举办，并由西湖欧米承办，SCIEX协办。

本期研讨会主题：Upleveling Proteomics to Better Understand and Detect Human Disease

直播时间：2023年4月27日（周四）8:00-9:30 PM 北京时间8:00-9:30 AM EST

报告专家：

Neil Kelleher

西北大学生命过程化学研究所（CLP）主任

西北大学Feinberg医学院分子生物科学系西北蛋白质组学实验室主任

西北大学Walter and Mary Elizabeth Glass 讲席教授

讨论嘉宾：

王冠博，Guanbo Wang

北京大学生物医学前沿

创新中心（BIOPIC）研究员

王方军，Fangjun Wang

中国科学院

大连化学物理研究所教授

李惠琳，Huilin Li

中山大学药学院教授

郭天南，Tiannan Guo

西湖大学长聘副教授

西湖实验室 iMarker Lab 主任

西湖欧米创始人

️Abstract:

Since the completion of the Human Genome Project, much has been made of the need to bridge the gap from genes and traits. As a key nexus for the many interacting ‘-omes’ (genome, transcriptome, proteome, metabolome, exposome, interactome, etc.), the proteome should offer a tight link between genotype and phenotype. 

Why then is proteomics challenged in this connection after 20 years of intense development? The proteoform concept offers a potential solution by considering protein composition with complete molecular specificity. Proteoforms, or all the precise molecular forms of a protein, capture all sources of variability in protein composition (i.e., SNPs, isoforms, post-translational modifications, etc.), and thus provide crucial insights into biological function of a protein by integrating primary sequence and post-translational modifications (e.g., enabling discovery of allele- or isoform-specific PTM patterns). This level of characterization is not possible at the peptide level, the unit of measurement of traditional ‘bottom-up’ proteomics, or the epitope level, the target of antibody-based techniques. 

Our group’s studies of disease-related genes have demonstrated the importance of proteoform measurement in clinically relevant protein targets such as KRAS (Adams et al., JBC, 2023), ApoA-1 (Wilkins et al., JAHA, 2021), and immunoproteoforms (Melani et al., Science, 2022). 

Now, “single molecule” mass spectrometry is poised to convert genes to proteoform signatures at a far faster rate.  The latest technologies for scalable and systematic mapping of proteoforms to promote human health will be described in this seminar. 

往期回顾详见：https://www.westlakeomics.com/conferences/