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样品: | 向日葵 | 项目: | 悬浮培养 |
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向日葵细胞悬浮培养 |
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The cultivation of plant cells continuously became more important in the last years due to a high amount of different substances which could be used by the pharmaceutical and cosmetic industry. However, during cultivation of plant suspension foam generation and shear stress occurs which may have negative effect on cell
growth. In addition, cell growth, aggregation and the release of exopolysaccarides lead to a continuous change of the rheological behaviour, which requires constant process adjustment.
Besides STR’s (stirred tank reactor), disposable (single‐use) systems such as the Biostat CultiBag RM, which is well established in cultivation volumes of 2 to 20 L, are used for plant cell cultivation. By inducing an orbitally shaking motion or a wave‐mixed motion, mixing is achieved and oxygen is transferred from the aerated reactor chamber into the culture medium.