X

您需要的产品资料:

选型配置
产品样品
使用说明
应用方案
公司录用
其他

个人信息:

提交
我的咨询

您好,欢迎您查看分析测试百科网,请问有什么帮助您的?

稍后再说 立即咨询
咨询列表
Abberior
X
头像

客服中心

如果企业客服不在线,也可拨打400电话联系。或者发布求购信息

400-6699-117转1000
发布求购 实验百科采购-随时微信沟通
扫码关注
实验采购百科
您现在所在的位置:首页 >> 仪器导购 >> 超分辨率显微镜>> RESCue STED 超分辨活细胞成像系统

RESCue STED 超分辨活细胞成像系统

tel: 400-6699-117 1000

Abberior超分辨率显微镜, Abberior RESCue STED超分辨活细胞成像 RESCue = REduction of State transition Cycles 所有的 Abberior STED 超分辨系统, 皆可升级附加 RESCue STED.

在线客服


RESCue STED技术的基本原理

RESCue (REduction of State transition Cycles) STED is an improved, photon-efficient STED imaging mode that significantly reduces the light dose sent onto your sample without compromising the resolution – STED imaging conditions designed for live-cell STED imaging!


· RESCue avoids unnecessary excitation and de-excitation cycles and thus reduces photobleaching of any fluorescent marker

· The reduction in excitation and de-excitation cycles is particularly beneficial for volume imaging with 3D STED as well as for time lapse STED imaging

· RESCue STED applies excitation and STED light only at positions where fluorescent markers (i.e. the target structure) is present. It shuts off the lasers at positions where exposure would not make sense (i.e. where no markers are found)

· The resolution of the image is maintained although the light dose is reduced down to 4 % compared to gated cw STED imaging and down to 20 % compared to pulsed STED imaging

· RESCue STED is a highly photon-efficient way of superresolution imaging while conventional STED applies the same light dose to any pixel no matter if markers (i.e. a target) are present or not

· RESCue can be applied with similar benefits also to confocal imaging


RESCue STED 与其他技术的曝光剂量比较简单的举例, Leica g-STED 的曝光剂量是Abberior RESCue-STED 的25倍 !

使用实务上, 曝光剂量愈少, 对样本(活细胞)的伤害愈小, 荧光也愈不会漂白 !


左边 : RESCue-STED                                              右边 : 传统的 gated-STED

经过 30 次的 RESCue-STED 扫描取像.                   经过 30 次的 gated –CW STED扫描取像,

STED 影像依然清晰可见                                         STED 影像因为重复曝光, 产生严重的荧光漂白, 影像效果极差.

因为几乎没有荧光漂白现象

RESCue STED应用

· RESCue STED 是 Abberior STED 的附加模块, 可以不影响超分辨的光学解析下, 大副降低光辐射剂量, 仅是过去的gated-cw-STED 成像技术的 4% 光剂量而已. 在既有新一代的 Pulsed STED 技术, 有了 RESCue STED 模块, 也仅是使用 20% 的光剂量而已.

· RESCue STED 成像技术, zei适宜于活细胞超分辨成像及 3D-STED 的超分辨立体成像

The blue color indicates at which sample positions the excitation and the STED laser were shut off in the RESCue STED imaging mode at the lower threshold. Note that the blue positions correlate with the positions where no marker (i.e. target structure) is present. 
Shown are Vero cells labelled with antibodies against nuclear pore complex subunits in the central channel of the complex and secondary antibodies coupled to Abberior STAR RED



RESCue STED in living yeast cells @ 595 nm.
Shown are Citrine-labelled eisosomes in living yeast cells (sample courtesy of Prof. Dr. S. Jakobs, University Medical Center G?ttingen). Note that only ~4 % of the light dose was applied during image generation (compared to gated cw STED).

相较于 Leica 的 gated CW STED ( TCS SP8 STED ),  Abberior RESCue STED 仅使用小于 4% 的光打到活细胞样本.

所以, 没有光漂白, 光毒害, 可以长时间做活细胞影像撷取


RESCue 3D STED @ 775 nm.

A 3D image stack of complete mammalian nucleus was recorded with RESCue 3D STED and conventional STED microscopy. The upper part of the figure shows xz sections of a 3D STED image stack. The lower part shows three planes (xy sections) of the 3D STED image stack. The position of the individual xy planes is labeled by A,B,C in the top images.

Shown are Vero cells labelled with antibodies against nuclear pore complex subunits in the central channel of the complex and secondary antibodies coupled to Abberior STAR RED


RESCue STED @ 595 nm.
Shown are Vero cells labelled with phalloidin coupled to Oregon Green 488.



厂家资料

地址:Abberior Instruments GmbH Hans-Adolf-Krebs-Weg 1 D-37077 Göttingen / Germany

电话:400-6699-117 转 1000

经销商

售后服务

我会维修/培训/做方法

如果您是一名工程师或者专业维修科学 仪器的服务商,都可参与登记,我们的平台 会为您的服务精确的定位并展示。

1该产品的品牌知名度如何?
2你对该产品的使用感受如何?
3该产品的性价比如何?
4该产品的售务如何?
查看
在线咨询

在线咨询时间:

周一至周五

早9:00 - 晚17:30

若您在周六周日咨询,请直接留言您所咨询的产品名称+联系人+电话